Results 61 to 70 of about 6,735,254 (244)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Förster Resonance Energy Transfer Measurements in Living Plant Cells

, 2021
Schmidtpott S, Seidel T. Förster Resonance Energy Transfer Measurements in Living Plant Cells. Journal of visualized experiments : JoVE. 2021;(172).Sensitized emission-based Forster resonance energy transfer (FRET) experiments are easily done but depend ...
Schmidtpott, Sonja, Seidel, Thorsten
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Molecular Rotors: From Newtonian Fluids to Functional Materials as Viscosity and Temperature Sensing Tools

Advanced Functional Materials, EarlyView.
Molecular rotors are fluorescent molecules that are sensitive to viscosity and temperature variations in the environment. They exhibit a direct correlation between fluorescence and viscosity within Newtonian fluids. However, this correlation becomes less straightforward in complex systems.
Flavia Di Scala, Alice Briole, Valerii Myndrul, Thomas J. Cleij, Hanne Diliën, Bérengère Abou, Bart van Grinsven   +6 more
wiley   +1 more source

Tetracyclic π‐Extended Multi‐Resonant TADF Emitters for Green PSF‐OLEDs With 37.7% EQE and BT.2020‐Compatible Top‐Emitting Devices

Advanced Functional Materials, EarlyView.
Ultra‐narrow green multi‐resonance thermally activated delayed fluorescence emitters have been developed by linear π‐extension strategy using tetracyclic naphtho[2,3‐b]benzofuran unit. The resulting phosphorescence‐sensitized OLEDs achieve a maximum external quantum efficiency of 37.7% in the bottom‐emitting architecture, while the complementary top ...
Eo Jin Jeon, Truong Thi Thuy, Nikita Abitkar, Paramasivam Palanisamy, Jiheon Kim, Myungsun Sim, Athithan Maheshwaran, Jang Hyuk Kwon   +7 more
wiley   +1 more source

Fast spontaneous emission and high Förster resonance energy transfer rate in hybrid organic/inorganic plasmonic nanostructures

, 2018
We report an experimental study of the plasmon-assisted spontaneous emission and the Förster resonance energy transfer between organic molecules and semiconductor colloidal quantum dots.
Asgar, Hassnain, Jacob, Liyan, Hoang, Thang B., Thang B. Hoang, Hassnain Asgar, Liyan Jacob   +5 more
core   +1 more source

Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential

Advanced Healthcare Materials, EarlyView.
This review examines how cellular behavior is regulated by mechanical cues transmitted through soft biomaterials, from single‐cell mechanosensing to tissue‐level adaptation. It highlights why physiological relevance, rather than model complexity alone, is critical for translational mechanobiology and introduces a scoring framework linking material ...
Mathias Polz, Manuel P. Kainz, Niklas Haider, Theresa Rienmüller, Kerstin Lenk, Rupert Kargl, Michele Terzano, Gerhard Sommer, Gerhard A. Holzapfel, Julia Fuchs   +9 more
wiley   +1 more source

Spectral and time-resolved photoluminescence of human platelets doped with platinum nanoparticles.

PLoS ONE, 2021
This paper describes a detailed study of spectral and time-resolved photoprocesses in human platelets and their complexes with platinum (Pt) nanoparticles (NPs).
Karina Matveeva, Andrey Zyubin, Elizaveta Demishkevich, Vladimir Rafalskiy, Ekaterina Moiseeva, Igor Kon, Anna Kundalevich, Viktoria Butova, Ilia Samusev   +8 more
doaj   +1 more source

Förster resonance energy transfer imaging in vivo with approximated radiative transfer equation [PDF]

, 2011
We describe a new light transport model, which was applied to three-dimensional lifetime imaging of Förster resonance energy transfer in mice in vivo. The model is an approximation to the radiative transfer equation and combines light diffusion and ray ...
McGinty, J, Hajnal, JV, Arridge, Simon R., Dominic J. Wells, Marzena Wylezinska-Arridge, James McGinty, Joseph V. Hajnal, Wells, DJ, Wylezinska-Arridge, M, Soloviev, VY, Sardini, A, Vadim Y. Soloviev, Simon R. Arridge, Wylezinska-Arridge, Marzena, Romain Laine, Stuckey, Daniel W., Soloviev, Vadim Y., Sardini, Alessandro, Laine, R, Stuckey, DW, Paul M. W. French, Daniel W. Stuckey, Alessandro Sardini, McGinty, James, Wells, Dominic J., French, PMW, French, Paul M. W., Hajnal, Jo; id_orcid, Laine, Romain, Arridge, SR   +29 more
core   +1 more source

Azaporphyrinoid‐Based Photo‐ and Electroactive Architectures for Advanced Functional Materials

Advanced Materials, EarlyView.
A long‐standing collaboration between the Torres and Guldi groups has yielded diverse azaporphyrinoid‐based donor‐acceptor nanohybrids with promising applications in solar energy conversion. This conspectus highlights key molecular platforms and structure‐function relationships that govern light and charge management, supporting the rational design of ...
Jorge Labella, Kobra Azizi, Dirk M. Guldi, Tomás Torres   +3 more
wiley   +1 more source