Results 41 to 50 of about 8,058,982 (114)

Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs

Biophysical Reports, 2021
Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and ...
Asaf Grupi, Zehavit Shapira, Shimon Yudovich, Nurit Degani-Katzav, Shimon Weiss   +4 more
doaj   +1 more source

Enhanced Förster Resonance Energy Transfer (FRET) on a Single Metal Particle

, 2016
We examined the effect of a metallic silver particle on Förster resonance energy transfer (FRET) between a nearby donor−acceptor pair. A donor-labeled oligonucleotide was chemically bound to a single silver particle, and then an acceptor-labeled ...
Joseph R. Lakowicz (24739), Yi Fu (315254), Jian Zhang (1682)   +2 more
core   +1 more source

A protein L-based immunodiagnostic approach utilizing time-resolved Förster resonance energy transfer. [PDF]

PLoS ONE, 2014
Chelated lanthanides such as europium (Eu) have uniquely long fluorescence emission half-lives permitting their use in time-resolved fluorescence (TRF) assays.
Satu Hepojoki, Visa Nurmi, Antti Vaheri, Klaus Hedman, Olli Vapalahti, Jussi Hepojoki   +5 more
doaj   +1 more source

ATP changes the fluorescence lifetime of cyan fluorescent protein via an interaction with His148 [PDF]

, 2010
Contains fulltext : 87235.pdf (Publisher’s version ) (Open Access)Recently, we described that ATP induces changes in YFP/CFP fluorescence intensities of Fluorescence Resonance Energy Transfer (FRET) sensors based on CFP-YFP.
Marieke Willemse, Jalink Kees, Fransen, J.A.M., Jalink, K., Sergey P Laptenok, Krogt, G. van der, Krogt, G., Jan Willem Borst, Laptenok Sergey P., Jack A M Fransen, Slijkhuis Rik, Slijkhuis, R., Willemse, M.P., Fransen Jack A. M., Jack A. M. Fransen, Laptenok, S., Willemse, M., Borst, J.W., Wieringa, B., Borst Jan Willem, Gerard van der Krogt, Rik Slijkhuis, Kees Jalink, Sergey P. Laptenok, van der Krogt Gerard, Laptenok, S.P., Be Wieringa, Wieringa Be, Willemse Marieke   +28 more
core   +3 more sources

Förster resonance energy transfer measurements of ryanodine receptor type 1 structure using a novel site-specific labeling method. [PDF]

PLoS ONE, 2009
While the static structure of the intracellular Ca(2+) release channel, the ryanodine receptor type 1 (RyR1) has been determined using cryo electron microscopy, relatively little is known concerning changes in RyR1 structure that accompany channel gating.
James D Fessenden
doaj   +1 more source

Protocol for deriving proximity, affinity, and stoichiometry of protein interactions using image-based quantitative two-hybrid FRET

STAR Protocols, 2023
Summary: Two-hybrid Förster resonance energy transfer (FRET) provides proximity, affinity, and stoichiometry information in binding interactions. We present an image-based approach that surpasses traditional two-hybrid FRET assays in precision and ...
Colin Feldmann, Michael Schänzler, Manu Ben-Johny, Christian Wahl-Schott   +3 more
doaj   +1 more source

Photophysical study of fused arene based dyes effective for Förster resonance energy transfer [PDF]

, 2022
This dissertation work investigates the non-radiative energy transfer process in a blend of donor and acceptor of fused arene-based hybrid fluorophore materials.
Hawkins, Matthew D., NC DOCKS at The University of North Carolina at Greensboro   +1 more
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Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen

Sensors, 2016
The integration of semiconductor quantum dots (QDs) into homogeneous Förster resonance energy transfer (FRET) immunoassay kits for clinical diagnostics can provide significant advantages concerning multiplexing and sensitivity.
Shashi Bhuckory, Olivier Lefebvre, Xue Qiu, Karl David Wegner, Niko Hildebrandt   +4 more
doaj   +1 more source

FRET Based Biosensor: Principle Applications Recent Advances and Challenges

Diagnostics, 2023
Förster resonance energy transfer (FRET)-based biosensors are being fabricated for specific detection of biomolecules or changes in the microenvironment.
Awadhesh Kumar Verma, Ashab Noumani, Amit K. Yadav, Pratima R. Solanki   +3 more
doaj   +1 more source

Synthesis of Molecular Rulers to Study Distance and Orientation Dependent Förster Resonance Energy Transfer (FRET) [PDF]

, 2019
Sahoo D. Synthesis of Molecular Rulers to Study Distance and Orientation Dependent Förster Resonance Energy Transfer (FRET).
Sahoo, Dhananjaya
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