Direct Imaging of the Energy-Transfer Enhancement between Two Dipoles in a Photonic Cavity
Photonic cavities are gathering a large amount of interest to enhance the energy transfer between two dipoles, with far-reaching consequences for applications in photovoltaics, lighting sources, and molecular biosensing.
Kaizad Rustomji +6 more
doaj +1 more source
Robust evaluation of intermolecular FRET using a large Stokes shift fluorophore as a donor
Fluorescence (or Förster) resonance energy transfer (FRET) is a straightforward and sensitive technique to evaluate molecular interactions. However, most of the popular FRET pairs suffer cross-excitation of the acceptor, which could lead to false ...
Carmen Santana-Calvo +3 more
doaj +1 more source
How structural disorder and polyelectrolyte properties of prothymosin α enable its large interactome
In this review, complexes of the fully disordered and highly negatively charged prothymosin α are collected, described, and discussed. Linked to its poly‐electrostatic character, prothymosin α engages a large interactome through multiple different binding modes ranging from fully disordered complexes to short linear motif (SLiM)‐driven interactions ...
Ketty C. Tamburrini +3 more
wiley +1 more source
Förster Resonance Energy Transfer between Quantum Dot Donors and Quantum Dot Acceptors
Förster (or fluorescence) resonance energy transfer amongst semiconductor quantum dots (QDs) is reviewed, with particular interest in biosensing applications.
Kenny F. Chou, Allison M. Dennis
doaj +1 more source
Efficient CO2 photoreduction enabled by the energy transfer pathway in metal‐organic framework
Many studies in metal‐organic frameworks (MOFs) aiming for high photocatalytic activity resort to self‐assembling both energy donor and acceptor building units in skeleton to achieve effective energy transfer, which, however, usually needs tedious ...
Hai‐Xiong Liu +9 more
doaj +1 more source
Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim +11 more
wiley +1 more source
Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs
Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and ...
Asaf Grupi +4 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Chiral dopants induce supramolecular chirality in an achiral polymer (PFO), generating spin‐polarized carriers via the chiral‐induced spin selectivity (CISS) and suggesting a possible contribution of Spin‐polarized carrier transport to circularly polarized electroluminescence (CPEL).
Kazuyuki Asahi +4 more
wiley +1 more source

