Results 11 to 20 of about 8,058,982 (114)

Fluorescence resonance energy transfer studies of protein interactions [PDF]

, 2008
This thesis presents an investigation of fluorescence resonance energy transfer (FRET) as a reporting signal for protein-protein interactions. Quantitative optical assays to measure protein binding, conjugation and deconjugation are developed and results
Martin, Sarah Friede
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Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Ankerhold, Richard, Richard Ankerhold, Drummen, Gregor P. C., Gregor P. C. Drummen, Hellen C. Ishikawa-Ankerhold, Ishikawa-Ankerhold, Hellen C.   +5 more
core   +1 more source

No better time to FRET: shedding light on host pathogen interactions [PDF]

, 2010
Understanding the spatio-temporal subversion of host cell signaling by bacterial virulence factors is key to combating infectious diseases. Following a recent study by Buntru and co-workers published in BMC Biology, we review how fluorescence (Forster ...
Leong, J.M., Goguen, J.D., Goguen, JD, Leong, JM, Hayward, RD, Hayward, Richard D.   +5 more
core   +1 more source

Linear approaches to intramolecular Förster Resonance Energy Transfer probe measurements for quantitative modeling [PDF]

, 2011
Numerous unimolecular, genetically-encoded Forster Resonance Energy Transfer (FRET) probes for monitoring biochemical activities in live cells have been developed over the past decade.
Kurt I. Anderson, Kida, K., Katarzyna Kida, Birtwistle, M.R., Kriegsheim, Alexander von, Anderson, K.I., Kida Katarzyna, von Kriegsheim, A., Walter Kolch, Anderson Kurt I., Kida, Katarzyna, Schwarz, J.P., Marc R Birtwistle, Birtwistle Marc R., Birtwistle, Marc R., Alexander von Kriegsheim, Kurt I Anderson, von Kriegsheim Alexander, Juliane P Schwarz, Schwarz Juliane P., Kolch, W., Marc R. Birtwistle, Kolch Walter, Juliane P. Schwarz, et al.   +24 more
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Structural Changes of Yellow Cameleon Domains Observed by Quantitative FRET Analysis and Polarized Fluorescence Correlation Spectroscopy [PDF]

, 2008
Förster resonance energy transfer (FRET) is a widely used method for monitoring interactions between or within biological macromolecules conjugated with suitable donor-acceptor pairs.
Laptenok, S., Visser, N.V., Kalinin, S., Hornen, H., Hoek, A., van, Kühnemuth, R., Borst, J.W., van Hoek, A., Westphal, A.H., Aker, J., Aker, J.C.M., Kyhnemuth, R., Laptenok, S.P., Seidel, C.A.M., Visser, A.J.W.G.   +14 more
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Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs [PDF]

, 2010
Förster resonance energy transfer (FRET) is a powerful method for obtaining information about small-scale lengths between biomacromolecules. Visible fluorescent proteins (VFPs) are widely used as spectrally different FRET pairs, where one VFP acts as a ...
Birch, D.J.S., Laptenok, S., Visser, N.V., Hoek, A., van, Laptenok, S.P., Borst, J.W., Visser, A.J.W.G., van Hoek, A., Brochon, J.C.   +8 more
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Energy transfer in complexes of water-soluble quantum dots and chlorin e6 molecules in different environments

Beilstein Journal of Nanotechnology, 2013
The photoexcitation energy transfer is found and investigated in complexes of CdSe/ZnS cationic quantum dots and chlorin e6 molecules formed by covalent bonding and electrostatic interaction in aqueous solution and in porous track membranes.
Irina V. Martynenko, Anna O. Orlova, Vladimir G. Maslov, Alexander V. Baranov, Anatoly V. Fedorov, Mikhail Artemyev   +5 more
doaj   +1 more source

FRET Ratiometric Nanoprobes for Nanoparticle Monitoring

Biosensors, 2021
Fluorescence labelling is often used for tracking nanoparticles, providing a convenient assay for monitoring nanoparticle drug delivery. However, it is difficult to be quantitative, as many factors affect the fluorescence intensity.
Guangze Yang, Yun Liu, Jisi Teng, Chun-Xia Zhao   +3 more
doaj   +1 more source

A Guide to Fluorescent Protein FRET Pairs

Sensors, 2016
Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most
Bryce T. Bajar, Emily S. Wang, Shu Zhang, Michael Z. Lin, Jun Chu   +4 more
doaj   +1 more source

QTR-FRET: Efficient background reduction technology in time-resolved förster resonance energy transfer assays [PDF]

, 2022
A novel homogeneous assay system QTR-FRET (Quencher modulated Time-Resolved Forster Resonance Energy Transfer) combining quenching resonance energy transfer (QRET) and time-resolved Forster resonance energy transfer (TR-FRET) was developed to reduce ...
Emmiliisa Vuorinen, Harri Härmä, Sakari Kulmala, Markku Syrjänpää, Qi Wang, Kari Kopra   +5 more
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