Results 31 to 40 of about 1,699 (151)

Detection of Mycobacterium avium ssp. paratuberculosis in cheese, milk powder and milk using IS900 and f57-based qPCR assays [PDF]

Journal of Applied Microbiology, 2010
To develop a quantitative PCR assay for sensitive and specific detection of Mycobacterium avium ssp. paratuberculosis (Map) in a range of dairy products.TaqMan(®) assays were designed to target the IS900 and f57 genetic elements of Map. Both real-time PCR assays were integrated with the Adiapure(®) Map DNA extraction kit and assessed separately for the
J.A. Donaghy, J. Johnston, M.T. Rowe
openaire   +2 more sources

Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd

Revista Colombiana de Ciencias Pecuarias, 2017
Background: paratuberculosis is a slow-developing infectious disease, characterized by chronic granulomatous enterocolitis. This disease has a variable incubation period from 6 months to over 15 years, and is caused by Mycobacterium avium subsp ...
Nathalia M. Correa Valencia, Nicolás F. Ramírez, Michael Bülte, Jorge A. Fernández Silva   +3 more
doaj   +1 more source

Goat Paratuberculosis: Experimental Model for the Evaluation of Mycobacterium Persistence in Raw Milk Cheese

Microorganisms, 2021
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of chronic proliferative enteritis found in ruminants, known as paratuberculosis (PTB).
Giulia Pagliasso, Alessia Di Blasio, Nicoletta Vitale, Angelo Romano, Lucia Decastelli, Antonio Quasso, Matteo Ricchi, Alessandro Dondo, Paolo Pastorino, Maria Silvia Gennero, Stefania Bergagna   +10 more
doaj   +1 more source

Impact of salt concentration on persistence of Mycobacterium avium subsp. paratuberculosis in Iranian UF white cheese [PDF]

Bihdāsht-i Mavādd-i Ghaz̠āyī, 2013
Mycobacterium avium subsp. paratuberculosis (Mycobacterium paratuberculosis) is considered as a potential significant public health threat due to its possible association with Crohn’s disease in humans.
Sh Hanifian, H Jodeiri
doaj  

In situ estimation of indigestible compounds contents in cattle feed and feces using bags made from different textiles Estimação in situ dos componentes indigestíveis em alimentos e fezes de bovinos com sacos de diferentes tecidos

Revista Brasileira de Zootecnia, 2011
The objective of this experiment was to evaluate estimates of concentration of indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) in samples of cattle feed and feces processed at ...
Tiago Neves Pereira Valente, Edenio Detmann, Sebastião de Campos Valadares Filho, Maura da Cunha, Augusto César de Queiroz, Cláudia Batista Sampaio   +5 more
doaj   +1 more source

Confirmation test of suspected Mycobacterium avium subspecies paratuberculosis (MAP) isolated using PCR F57

Jurnal Ilmu Ternak dan Veteriner, 2014
Seropositive and isolate suspected as Mycobacterium avium subspecies paratuberculosis (MAP) was detected at dairy cows in West Java. This bacteria causes Johne’s disease (JD) and potentially becomes a new emerging disease for Indonesian dairy cows.
Widagdo Sri Nugroho, Rahmat Setya Adji, Aeth Wahyuni   +2 more
openaire   +1 more source

Simulation of variations in the composition of samples in the evaluation of neutral detergent fiber contents by using cellulose standard in filter bags made from different textiles

Revista Brasileira de Zootecnia, 2011
The objective of this study was to evaluate the efficiency of using nylon textiles (50 μm), F57 (Ankom®) and non-woven textile (NWT - 100 g/m²) on laboratory evaluation of neutral detergent fiber (NDF) by using quantitative filter paper as purified ...
Tiago Neves Pereira Valente, Edenio Detmann, Sebastião de Campos Valadares Filho, Mário Fonseca Paulino, Janderson Florêncio Figueiras, Marjorrie Augusto de Souza   +5 more
doaj   +1 more source

Development of an F57 Sequence-Based Real-Time PCR Assay for Detection ofMycobacterium aviumsubsp.paratuberculosisin Milk [PDF]

Applied and Environmental Microbiology, 2005
ABSTRACTA light cycler-based real-time PCR (LC-PCR) assay that amplifies the F57 sequence ofMycobacterium aviumsubsp.paratuberculosiswas developed. This assay also includes an internal amplification control template to monitor the amplification conditions in each reaction.
T, Tasara, R, Stephan
openaire   +2 more sources

Evaluation of neutral detergent fiber contents in forages, concentrates and cattle feces ground at different particle sizes and using bags made from different textiles Avaliação dos teores de fibra em detergente neutro em forragens, concentrados e fezes bovinas moídas em diferentes tamanhos e em sacos de diferentes tecidos

Revista Brasileira de Zootecnia, 2011
Two experiments were carried out to evaluate the effects of particles sizes on contents of neutral detergent fiber (NDF) by using nylon bags F57 (Ankom®) and non-woven textile (NWT 100 g/m²).
Tiago Neves Pereira Valente, Edenio Detmann, Sebastião de Campos Valadares Filho, Augusto César de Queiroz, Cláudia Batista Sampaio, Daiany Iris Gomes   +5 more
doaj   +1 more source

Allosteric mechanism for KCNE1 modulation of KCNQ1 potassium channel activation

eLife, 2020
The function of the voltage-gated KCNQ1 potassium channel is regulated by co-assembly with KCNE auxiliary subunits. KCNQ1-KCNE1 channels generate the slow delayed rectifier current, IKs, which contributes to the repolarization phase of the cardiac action
Georg Kuenze, Carlos G Vanoye, Reshma R Desai, Sneha Adusumilli, Kathryn R Brewer, Hope Woods, Eli F McDonald, Charles R Sanders, Alfred L George Jr, Jens Meiler   +9 more
doaj   +1 more source