Results 71 to 80 of about 3,131,355 (307)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

We are all one together : peer educators\u27 views about falls prevention education for community-dwelling older adults - a qualitative study [PDF]

, 2015
Background: Falls are common in older people. Despite strong evidence for effective falls prevention strategies, there appears to be limited translation of these strategies from research to clinical practice.
A Bandura, A Dickinson, A-M Hill, A-M Hill, AJ Milat, AJ Onwuegbuzie, Anne-Marie Hill, BG Glaser, C Abraham, C Bradley, DH Brundage, DR Krathwohl, E Dale, E Robson, EA Skinner, F Bunn, Fiona Farringdon, G Foster, H Deery, J Collins, J Dolinis, J Dollard, J Gorham, J Sciacca, JM Morse, JM Simoni, JP Lalley, K Hughes, KE Mosack, Keith D Hill, L Yardley, LA Klein, LD Gillespie, Linda Khong, M Knowles, M Shiner, MB Miles, MQ Patton, NK Denzin, NM Peel, PM Davidson, S Michie, S Michie, S Vernon, SJ Snodgrass, SR Nyman, T Allen, T Karwalajtys, TP Haines, V Braun   +49 more
core   +2 more sources

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration

Biology Open, 2016
Cystine and cysteine are important molecules for pathways such as redox signaling and regulation, and thus identifying cellular deficits upon deletion of the Saccharomyces cerevisiae cystine transporter Ers1p allows for a further understanding of cystine
Jessica A. Simpkins, Kirby E. Rickel, Marianna Madeo, Bethany A. Ahlers, Gabriel B. Carlisle, Heidi J. Nelson, Andrew L. Cardillo, Emily A. Weber, Peter F. Vitiello, David A. Pearce, Seasson P. Vitiello   +10 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Guideline‐directed medical therapy implementation during hospitalization for cardiogenic shock

ESC Heart Failure
Aims Despite significant morbidity and mortality, recent advances in cardiogenic shock (CS) management have been associated with increased survival. However, little is known regarding the management of patients who survive CS with heart failure (HF) with
Matthew G. Dimond, Carolyn M. Rosner, Seiyon Ben Lee, Unique Shakoor, Taraneh Samadani, Wayne B. Batchelor, Abdulla A. Damluji, Shashank S. Desai, Kelly C. Epps, M. Casey Flanagan, Hala Moukhachen, Anika Raja, Matthew W. Sherwood, Ramesh Singh, Palak Shah, Daniel Tang, Behnam N. Tehrani, Alexander G. Truesdell, Karl D. Young, Mona Fiuzat, Christopher M. O'Connor, Shashank S. Sinha, Mitchell A. Psotka   +22 more
doaj   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

FEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

Why human connection is the true metric of research success

FEBS Open Bio, EarlyView.
Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan, Mattias Wei Ren Kon, Xavier Coumoul, Fun Man Fung   +3 more
wiley   +1 more source

Enhancing casein micelle dissociation in diluted skim milk systems using combined processing techniques

Journal of Dairy Science
: The present work aims to evaluate the dissociation of casein micelles in diluted skim milk (SM) systems after undergoing solvent- or emulsifying salt-based dissociation coupled with ultra-high-pressure homogenization (UHPH).
D.R. Stroinski, K. Petersen, G.E. Lewis
doaj   +1 more source