Results 21 to 30 of about 153,465 (260)

False negative diagnostic errors with polymerase chain reaction for the detection of cryptococcal meningoencephalitis [PDF]

Medical Mycology, 2019
Abstract The accuracy of the BioFire FilmArray Meningitis/Encephalitis (ME) panel for the identification of Cryptococcus has recently been called into question. The primary objective of this study was to assess the agreement between the BioFire ME polymerase chain reaction (PCR) and other markers of cryptococcal infection.
Matthew A Krolikowski, Whitney D Krolikowski, Paras D Patel, Cameron G Lanier, Paul O Lewis   +4 more
openaire   +3 more sources

An ELISA protocol to improve the accuracy and reliability of serological antibody assays

MethodsX, 2017
To assay serum antibodies by indirect ELISA, it is critical to eliminate a variety of false positive and negative reactions attributed to the principle.
Takaki Waritani, Jessica Chang, Bonnie McKinney, Kuniaki Terato   +3 more
doaj   +1 more source

Association between False Positive, False Negative, High-Background Cases and Humidity in One Lambda LABTypeTM HLA-DR Typing

Korean Journal of Clinical Laboratory Science, 2023
Human leukocyte antigen (HLA) typing was performed in the diagnostic immunology laboratory of the Seoul National University Hospital. Among 611 HLA-DR tests, specific bead reactions suspected of being false positive and false negative in Lot 20 reagents ...
Hyang Son AN, Minsung SOHN
doaj   +1 more source

Non-Specific Binding and Cross-Reaction of ELISA: A Case Study of Porcine Hemoglobin Detection

Foods, 2021
Different types of enzyme-linked immunosorbent assays (ELISA) have been widely used to control food safety and quality. To develop an accurate and reproducible ELISA, false immunodetection results caused by non-specific binding (NSB) and cross-reaction ...
Xingyi Jiang, Meng Wu, Jonathan Albo, Qinchun Rao   +3 more
doaj   +1 more source

False negative polymerase chain reaction on cerebrospinal fluid samples in tuberculous meningitis [PDF]

Journal of Neurology, Neurosurgery & Psychiatry, 1999
There have been few studies in the literature concerned solely with the use of the polymerase chain reaction (PCR) to identify Mycobacterium tuberculosis DNA directly from CSF.1-4 These studies suggest that in some cases, PCR may be more sensitive than culture; however, in the largest study, performed …
D M, Gascoyne-Binzi, P M, Hawkey
openaire   +2 more sources

Comparative scores of ABO and Rh hemagglutination reactions

Medical Journal of Dr. D.Y. Patil Vidyapeeth, 2022
Background: Blood group determination is done by the slide test and tube test. Grading/scoring of hemagglutination reactions is performed through the naked eye and microscopy to detect spurious (false positive and negative) reactions.
Prashant Devidas Khuje, Mahesh S Karandikar, Bharti U Sable   +2 more
doaj   +1 more source

Problems Encountered with Nested PCR to Diagnosis of Phytoplasmas

Majallah-i ḥifāẓat-i giyāhān, 2016
Introduction: Phytoplasmas are phytopathogenic bacteria without cell walls that can be found in plant phloem, have been found associated with numerous diseases of plants worldwide.
C. Moslemkhani, S. Baghaee Ravari, P. Khodaygan   +2 more
doaj   +1 more source

In vitro diagnostics of coronavirus disease 2019: Technologies and application

Journal of Microbiology, Immunology and Infection, 2021
Laboratory-based diagnostic measures including virological and serological tests are essential for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Chih-Cheng Lai, Cheng-Yi Wang, Wen-Chien Ko, Po-Ren Hsueh   +3 more
doaj   +1 more source

Weight of evidence approach using a TK gene mutation assay with human TK6 cells for follow-up of positive results in Ames tests: a collaborative study by MMS/JEMS

Genes and Environment, 2021
Background Conflicting results between bacterial mutagenicity tests (the Ames test) and mammalian carcinogenicity tests might be due to species differences in metabolism, genome structure, and DNA repair systems. Mutagenicity assays using human cells are
Manabu Yasui, Takayuki Fukuda, Akiko Ukai, Jiro Maniwa, Tadashi Imamura, Tsuneo Hashizume, Haruna Yamamoto, Kaori Shibuya, Kazunori Narumi, Yohei Fujiishi, Emiko Okada, Saori Fujishima, Mika Yamamoto, Naoko Otani, Maki Nakamura, Ryoichi Nishimura, Maya Ueda, Masayuki Mishima, Kaori Matsuzaki, Akira Takeiri, Kenji Tanaka, Yuki Okada, Munehiro Nakagawa, Shuichi Hamada, Akihiko Kajikawa, Hiroshi Honda, Jun Adachi, Kentaro Misaki, Kumiko Ogawa, Masamitsu Honma   +29 more
doaj   +1 more source

Analysis of False-negative Findings of the Incomparable Accuracy and Swiftness of Flow Cytometric Diagnosis of Primary Central Nervous System Lymphoma

Neurologia Medico-Chirurgica, 2023
Primary central nervous system lymphoma (PCNSL), a relatively rare brain tumor, bears a dire prognosis. On occasion, the rapid progression of the tumor makes immediate diagnosis and initiation of therapy imperative.
Hayato TAKEUCHI, Tohru INABA, Yukiko SHISHIDO-HARA, Taku TSUKAMOTO, Shinsuke MIZUTANI, Takanari OKAMOTO, Seisuke TANIGAWA, Takumi YAMANAKA, Yoshinobu TAKAHASHI, Eiichi KONISHI, Junya KURODA, Naoya HASHIMOTO   +11 more
doaj   +1 more source