Results 51 to 60 of about 537,139 (296)

metaSHARK: software for automated metabolic network prediction from DNA sequence and its application to the genomes of Plasmodium falciparum and Eimeria tenella [PDF]

, 2005
The metabolic SearcH And Reconstruction Kit (metaSHARK) is a new fully automated software package for the detection of enzyme-encoding genes within unannotated genome data and their visualization in the context of the surrounding metabolic network ...
McConkey, G.A., Pinney, J.W., Shirley, M.W., Westhead, D.R.   +3 more
core   +2 more sources

False negative results from using common PCR reagents

BMC Research Notes, 2011
Background The sensitivity of the PCR reaction makes it ideal for use when identifying potentially novel viral infections in human disease. Unfortunately, this same sensitivity also leaves this popular technique open to potential contamination with ...
Atwood Allison A, Cummings Jessica L, Sobek Kathryn M, Bacich Dean J, O'Keefe Denise S   +4 more
doaj   +1 more source

Molecular analysis of PKU-associated PAH mutations: a fast and simple genotyping test [PDF]

, 2018
: Neonatal screening for phenylketonuria (PKU, OMIM: 261600) was introduced at the end of the 1960s. We developed a rapid and simple molecular test for the most frequent phenylalanine hydroxylase (PAH, Gene ID: 5053) mutations.
Antonio Angeloni, ARTIOLA, CRISTIANA, CARDUCCI, Carla, CARDUCCI, Claudia, D'AMICI, SIRIO, GIOVANNIELLO, TERESA, Manuela Tolve, PASQUALI, Amelia, PIZZUTI, Antonio, Vincenzo Leuzzi   +9 more
core   +1 more source

Diagnostics of cutaneous adverse drug reactions: evaluation of patch tests, lymphocyte transformation tests, and drug provocation tests

Journal of Cutaneous Immunology and Allergy
BackgroundPatch tests (PT) and/or lymphocyte transformation tests (LTT) are typically performed, when diagnosed with cutaneous adverse drug reactions (CADR). However, their positivity rates can vary depending on the rash type.
Tomomu Ishikawa, Risa Tamagawa-Mineoka, Risa Tamagawa-Mineoka, Koji Masuda, Norito Katoh, Takeshi Fukumoto   +5 more
doaj   +1 more source

Monoclonal and polyclonal antibodies for ante- and post-mortem detection of PrPSc in sheep

Semina: Ciências Agrárias, 2015
Scrapie is a disease that affects sheep and goats and is characterized by the accumulation of an abnormal isoform (PrPSc) of the cellular prion protein, PrPC, in the central nervous system (CNS) and in lymphoid tissues.
Michele Dietrich Moura Costa , Lorenzo González, Caroline Pinto de Andrade , David Driemeier, Jair Rodini Engracia Filho , Cristina Santos Sotomaior   +5 more
doaj   +1 more source

Successful identification of culprit drugs of perioperative anaphylaxis by repeated skin testing after negative first skin tests in a patient with a long distant history of perioperative anaphylaxis

Heliyon, 2021
Background: Perioperative anaphylaxis is a severe immediate hypersensitivity reaction to drugs administered in immediate temporal association to surgical procedures. The European Academy of Allergy and Clinical Immunology recommends allergologic tests be
Wasurat Sungworn, Orathai Theankeaw, Aree Jameekornrak Taweechue, Chamard Wongsa, Torpong Thongngarm, Mongkhon Sompornrattanaphan   +5 more
doaj   +1 more source

Autocatalytic sets in a partitioned biochemical network [PDF]

, 2013
In previous work, RAF theory has been developed as a tool for making theoretical progress on the origin of life question, providing insight into the structure and occurrence of self-sustaining and collectively autocatalytic sets within catalytic polymer ...
Hordijk, Wim, Smith, Joshua I, Steel, Mike   +2 more
core   +2 more sources

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

Strategy for Controlling Preferential Amplification and Avoiding False Negatives in PCR Typing

BioTechniques, 1996
The use of the PCR method for routine testing has increased dramatically during recent years. Most assays involve coamplification either of an internal control, of several alleles at a given locus or of a variety of bands produced by low-stringency ...
Thomas Weissensteiner, Jerry S. Lanchbury   +1 more
doaj   +1 more source

Colorimetric RT-LAMP SARS-CoV-2 diagnostic sensitivity relies on color interpretation and viral load

Scientific Reports, 2021
The use of RT-LAMP (reverse transcriptase—loop mediated isothermal amplification) has been considered as a promising point-of-care method to diagnose COVID-19.
Mateus Nóbrega Aoki, Bruna de Oliveira Coelho, Luiz Gustavo Bentim Góes, Paola Minoprio, Edison Luiz Durigon, Luis Gustavo Morello, Fabricio Klerynton Marchini, Irina Natassja Riediger, Maria do Carmo Debur, Helder I. Nakaya, Lucas Blanes   +10 more
doaj   +1 more source