Results 31 to 40 of about 267,301 (260)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Influence of Tularemia Microbe of Different Subspecies on the Functional Activity of Immunocompetent Cells of Experimental Animals

Проблемы особо опасных инфекций, 2010
Analysis of molecular-biological mechanisms of patho-, immuno- and morphogenesis allowed to determine the role of bactericidal mechanisms (oxygen-, nitroxide-related and oxygen-free) of tularemia microbe phagocytosis by immunophagocytic system cells ...
V. I. Dubrovina, S. A. Tatarnikov, Zh. A. Konovalova, V. V. Voitkova, A. V. Mazepa, S. V. Lukiyanova, V. N. Rychkova, A. I. Belkov, T. T. Shkaruba   +8 more
doaj   +1 more source

Metabolism features and milk microbiota of cows with mastitis in the Amur Oblast

Ветеринария сегодня, 2023
The effective management practices of dairy farming are inextricably linked with the production of high quality milk, while the mastitis is one of the causes of reductions in milk yields and quality.
M. E. Ostyakova, I. S. Shulga, V. K. Irkhina, K. S. Kositsyna, N. S. Golaydo   +4 more
doaj   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Peculiarities of the Effect of the Lipopolysaccharide of Tularemia Microbe of Different Subspecies upon Metabolic Activity of Phagocytes in Vitro

Проблемы особо опасных инфекций, 2011
Experimental preparations of the lipopolysaccharide (LPS) of Francisella tularensis of four subspecies (tularensis, holarctica, mediasiatica, novicida) were obtained using Tween extraction method.
V. I. Dubrovina, Zh. A. Konovalova, V. B. Nikolaev, V. V. Voitkova, S. A. Tatarnikov, A. V. Mazepa, E. Yu. Markov, Yu. O. Popova, O. V. Yur`eva   +8 more
doaj   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

The Role of Brucella abortus I-206 Thermoextracts in L- and S-form in Shaping the Immune Response in White Mice

Эпидемиология и вакцинопрофилактика, 2019
Background. Brucellosis is an acute infectious-allergic zoonotic disease in which sick farm animals are the main source of infection for humans. Immunization of animals and people on the territory of Russia is carried out by live vaccines, which, having ...
V. I. Dubrovina, T. P. Starovoitova, S. A. Vityazeva, N. L. Barannikova, T. A. Ivanova, G. B. Mukhturgin, A. V. Gromova, G. Yu. Sorkina, S. V. Balakhonov   +8 more
doaj   +1 more source

The Effectiveness of MALDI ToF Mass Spectrometry in Identification of Francisella tularensis Strains

Проблемы особо опасных инфекций, 2022
The aim of the study was to evaluate the effectiveness of MALDI‑ToF mass spectrometry in the identification of collection and newly isolated strains of tularemia pathogen using the database “Protein profiles of mass spectra of microorganisms belonging to
A. K. Syngeeva, A. S. Ostyak, E. S. Kulikalova, A. V. Mazepa, K. V. Naumova, S. V. Balakhonov   +5 more
doaj   +1 more source

Why human connection is the true metric of research success

FEBS Open Bio, EarlyView.
Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan, Mattias Wei Ren Kon, Xavier Coumoul, Fun Man Fung   +3 more
wiley   +1 more source

Results of Work of the Specialized Anti-Epidemic Teams of the Irkutsk Research Anti-Plague Institute in the Amur Region, Khabarovsk Territory and the Jewish Autonomous Region in 2013

Проблемы особо опасных инфекций, 2014
Displayed are the major results of work of the specialized anti-epidemic teams from Irkutsk Research Anti-Plague Institute, aimed at the provision for sanitary-epidemiological welfare of the population in the Amur Region, Khabarovsk Territory and the ...
S. V. Balakhonov, S. A. Kosilko, A. K. Noskov, L. M. Mikhailov, M. V. Chesnokova, E. S. Kulikalova, V. A. Vishnyakov, N. V. Breneva, N. G. Gefan   +8 more
doaj   +1 more source