Results 121 to 130 of about 7,553,650 (283)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Presaturated iron‐core fault current limiters for MVDC power system applications
IET Electric Power ApplicationsThis paper presents design and dynamic performance investigation of a 25kV full‐scale, modular topology (arranged on the sides of a regular hexagon), medium voltage direct current (MVDC) permanent magnet (PM) biased presaturated fault current limiter ...
Ibrahim A. Metwally, Mohamed Eladawy
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
FEATURES OF OPERATION OF A SUPERCONDUCTING CURRENT LIMITER AT THE SUDDEN SHORT CIRCUIT
Electrical engineering & Electromechanics, 2014 In the article the fault current limiter of inductive type with high-temperature superconducting coil and screen is considered. Main features of transient at occurrence of short circuit are analyzed.
V.G. Dan'ko , E.V. Goncharov
doaj
EAI Endorsed Transactions on Energy WebThis paper presents a voltage compensation-type current limiter based on a hybrid distribution transformer (HDT) structure, which integrates a three-winding transformer, AC/DC converter, DC/AC converter, current-limiting unit, bypass switches, and a ...
Ruiayo Wang +4 more
doaj +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
FEBS Open Bio, EarlyView.Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar +11 more
wiley +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
FEBS Open Bio, EarlyView.Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells
FEBS Open Bio, EarlyView.BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley +1 more source
On Fault Current Limiting and Specification of Superconducting Fault Current Limiters
IEEJ Transactions on Power and Energy, 1994 Seiji Sekine, Tanzo Nitta
openaire +2 more sources