Results 61 to 70 of about 3,154,738 (335)

Ultrafast Photoexcitation Induced Nonthermal Lattice Expansion in 2D Perovskite

open access: yesAdvanced Science
Light‐induced lattice change of perovskites is found to be critical for device performance. However, the underlying mechanism is still not fully understood.
Xiangyu Chen   +4 more
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

Femtosecond Laser Assisted Cataract Surgery in White Cataract

open access: yesDelhi Journal of Ophthalmology, 2015
Aim: To demonstrate application of femtosecond laser technology in patients with white cataract. Methods: Video-based description of successful management of intumescent white cataract with femtosecond laser technology.
Jeewan Singh Titiyal, Tarun Arora
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

open access: yesFEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer   +7 more
wiley   +1 more source

Femtosecond optoinjection of intact tobacco BY-2 cells using a reconfigurable photoporation platform [PDF]

open access: yes, 2013
A tightly-focused ultrashort pulsed laser beam incident upon a cell membrane has previously been shown to transiently increase cell membrane permeability while maintaining the viability of the cell, a technique known as photoporation.
Torrance Lesley   +43 more
core   +2 more sources

Targeted transfection by femtosecond laser

open access: yesNature, 2002
The challenge for successful delivery of foreign DNA into cells in vitro, a key technique in cell and molecular biology with important biomedical implications, is to improve transfection efficiency while leaving the cell's architecture intact. Here we show that a variety of mammalian cells can be directly transfected with DNA without perturbing their ...
Tirlapur, U, König, K
openaire   +4 more sources

Uniform Amorphization of Crystalline Silicon Surfaces Enabled by Deep Ultraviolet Femtosecond Laser Pulses

open access: yesAdvanced Engineering Materials, EarlyView.
Deep‐UV (258 nm) femtosecond pulses enable uniform amorphous silicon writing on Si(100)/(111) with a six‐fold larger fluence amorphization window than NIR methods. Optimized fluence and overlap yield 20–45 nm uniform and continuous amorphous layers. Microscopy shows sharp interfaces, and real‐time reflectivity reveals nanosecond melt–resolidification ...
Wissal Benali   +5 more
wiley   +1 more source

Mathematical model оf laser radiation femtosecond interaction with human skin

open access: yesJournal of Biomedical Photonics & Engineering, 2016
We present a mathematical model of linear and nonlinear processes that occur under the action of femtosecond laser radiation on the cutaneous covering.
Pavel Rogov, Viktor Bespalov
doaj   +1 more source

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