Redox-dependent hydrogen-bond network rearrangement of ferredoxin-NADP<sup>+</sup> reductase revealed by high-resolution X-ray and neutron crystallography. [PDF]
High‐resolution X‐ray and neutron crystallography reveal redox‐dependent rearrangements in the hydrogen‐bond network of ferredoxin–NADP+ reductase (FNR), highlighting structural changes around the FAD cofactor and shifts in water molecules that underpin its electron‐transfer mechanism.
Uenaka M+7 more
europepmc +2 more sources
Methanol and Carbon Monoxide Metabolism of the Thermophile Moorella caeni. [PDF]
An omics approach was used to elucidate the C1 metabolism of Moorella caeni DSM 21394 T, which exhibits acetogenic metabolism on methanol and hydrogenogenic metabolism on CO. Our results suggest that respiratory Complex I serves as the primary respiratory enzyme in methanol‐grown cells.
Vecchini Santaella NA+2 more
europepmc +2 more sources
How Small Proteins Adjust the Metabolism of Cyanobacteria Under Stress: The Role of Small Proteins in Cyanobacterial Stress Responses. [PDF]
Cyanobacteria are powered by oxygenic photosynthesis located in extensive internal membrane systems, the thylakoids. Using carboxysomes, they assimilate organic from inorganic carbon. The integration of photosynthesis, carbon fixation, assimilation of nitrogen, and other nutrients involves several small proteins.
Kraus A, Hess WR.
europepmc +2 more sources
Multidimensional Engineering of Escherichia coli for Efficient Adipic Acid Synthesis From Cyclohexane. [PDF]
An engineered Escherichia coli is developed to efficiently produce adipic acid (AA) from cyclohexanol, KA oil (a mixture of cyclohexanol and cyclohexanone), or cyclohexane through a combination of enzyme screening, protein fusion, and genome editing techniques. This approach has achieved the highest recorded productivity for biological AA production to
Wang F+6 more
europepmc +2 more sources
Two Key Ferredoxins for Nitrogen Fixation Have Different Specificities and Biophysical Properties
Two ferredoxins, FdC and FdN, are collectively essential for the process of nitrogen fixation by the iron nitrogenase in R. capsulatus. We explore the biophysical factors, through spectroscopic and structural characterizations, which determine ferredoxin functionality and pathway specificity. The structure of FdC (PDB: 9I2A) and model of FdN (AF‐D5ARY6)
Holly Addison+5 more
wiley +1 more source
Summary Flavodiiron proteins (FDPs) catalyse light‐dependent reduction of oxygen to water in photosynthetic organisms, creating an electron sink on the acceptor side of Photosystem I that protects the photosynthetic apparatus. However, ambiguity about the electron donor(s) remains, and the molecular mechanisms regulating FDP activity have remained ...
Lauri Nikkanen+9 more
wiley +1 more source
The thermophilic acetogenic model organism Moorella thermoacetica can utilize lactate as substrate. The formation of lactate by reduction of pyruvate is energetically favorable, but the reverse reaction, the oxidation of lactate to pyruvate with NAD+ as electron acceptor is challenging.
Florian P. Rosenbaum, Volker Müller
wiley +1 more source
Building Localized NADP(H) Recycling Circuits to Advance Enzyme Cascadetronics
A consequence of the high catalytic activities and selectivities of many enzymes is that cascades enclosed within a mesoporous conducting material using NADP(H) and intermediates as internal current carriers display the almost instantaneous information‐transfer properties usually associated with electronic circuits.
Ryan A. Herold+2 more
wiley +2 more sources
Design, Synthesis, and Application of Immobilized Enzymes on Artificial Porous Materials
This review comprehensively summarizes the advances in research for immobilized enzymes on artificial porous materials. It highlights the advantages of bottom‐up strategy, post‐synthesis modification and composite method of metal‐organic frameworks (MOFs), covalent organic frameworks (COFs) and hydrogen‐bonded organic frameworks (HOFs) and emphasizes ...
Lu Ran+4 more
wiley +1 more source
This research highlights the unique responses of perennial tree species, Populus trichocarpa, P. deltoides, and their F1 hybrid to heat stress after priming. P. deltoides demonstrated improved photosynthetic efficiency and distinct epigenetic signatures, while P.
Biruk A. Feyissa+9 more
wiley +1 more source