Results 21 to 30 of about 4,441 (206)

Characterization of a feruloyl esterase from Lactobacillus plantarum. [PDF]

Appl Environ Microbiol, 2013
ABSTRACT Lactobacillus plantarum is frequently found in the fermentation of plant-derived food products, where hydroxycinnamoyl esters are abundant. L. plantarum WCFS1 cultures were unable to hydrolyze hydroxycinnamoyl esters; however, cell extracts from the strain partially hydrolyze methyl ...
Esteban-Torres M, Reverón I, Mancheño JM, de Las Rivas B, Muñoz R.   +4 more
europepmc   +7 more sources

Feruloyl Esterases for Biorefineries: Subfamily Classified Specificity for Natural Substrates [PDF]

Frontiers in Bioengineering and Biotechnology, 2020
Feruloyl esterases (FAEs) have an important role in the enzymatic conversion of lignocellulosic biomass by decoupling plant cell wall polysaccharides and lignin. Moreover, FAEs release anti-oxidative hydroxycinnamic acids (HCAs) from biomass. As a plethora of FAE candidates were found in fungal genomes, FAE classification related to substrate ...
Emilie N. Underlin, Emilie N. Underlin, Matthias Frommhagen, Adiphol Dilokpimol, Gijs van Erven, Ronald P. de Vries, Mirjam A. Kabel   +6 more
openaire   +8 more sources

Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases

Frontiers in Microbiology, 2020
Construction of recombinant Escherichia coli strains carrying feruloyl esterase genes for secretory expression offers an attractive way to facilitate enzyme purification and one-step production of ferulic acid from agricultural waste.
Zhenshang Xu, Zhenshang Xu, Jian Kong, Susu Zhang, Ting Wang, Ting Wang, Xinli Liu, Xinli Liu   +7 more
doaj   +1 more source

A novel esterase from a soil metagenomic library displaying a broad substrate range

AMB Express, 2021
A novel esterase gene was isolated from a soil metagenomic library. The gene encoded a protein of 520 amino acids which contained a 21 aa signal peptide.
Jian Yao, Lun Gui, Shaocheng Yin
doaj   +1 more source

Penicillium chrysogenum as a fungal factory for feruloyl esterases

Applied Microbiology and Biotechnology, 2023
Plant biomass is a promising substrate for biorefinery, as well as a source of bioactive compounds, platform chemicals, and precursors with multiple industrial applications. These applications depend on the hydrolysis of its recalcitrant structure.
García-Calvo, Laura, Rodríguez-Castro, Raquel, Ullán, Ricardo V., Albillos, Silvia M., Fernández-Aguado, Marta, Vicente, Cláudia M., Degnes, Kristin F., Sletta, Håvard, Barreiro, Carlos   +8 more
openaire   +6 more sources

Regioselective chemoenzymatic syntheses of ferulate conjugates as chromogenic substrates for feruloyl esterases

Beilstein Journal of Organic Chemistry, 2021
Generally, carbohydrate-active enzymes are studied using chromogenic substrates that provide quick and easy color-based detection of enzyme-mediated hydrolysis.
Olga Gherbovet, Fernando Ferreira, Apolline Clément, Mélanie Ragon, Julien Durand, Sophie Bozonnet, Michael J. O'Donohue, Régis Fauré   +7 more
doaj   +1 more source

Enzyme Production Potential of Penicillium oxalicum M1816 and Its Application in Ferulic Acid Production

Foods, 2021
The present study focused on isolating an efficient enzyme production microorganism for ferulic acid (FA) production from wheat bran. A wild-type cellulase-, xylanase-, and feruloyl esterase-producing strain was isolated and identified as Penicillium ...
Jing Zhang, Shuangping Liu, Hailong Sun, Zhengfei Jiang, Zhilei Zhou, Xiao Han, Yongxiang Zhou, Honggen Sun, Weibiao Zhou, Jian Mao   +9 more
doaj   +1 more source

Discovering PETases: An Interlink Between Engineering Enzymes and Microbiomes. [PDF]

Environ Microbiol
In this review‐type article, we discuss some recent trends in the identification of polyethylene terephthalate‐degrading enzymes (PETases) from nature. We highlight that the discovery of novel PETases can be improved by an interlink between reshaping microbiomes and engineering proteins.
Jiménez DJ, Rosado AS.
europepmc   +2 more sources

Optimization of solid-state fermentation conditions for feruloyl esterase production by Aspergillus awamori [PDF]

Zhongguo niangzao
Using feruloyl esterase activity as the response value,the conditions for producing feruloyl esterase by Aspergillus awamori were optimized through single factor tests and response surface experiments.The results showed that the optimal fermentation ...
ZHANG Ting, LIU Xiying, CHEN Tao
doaj   +1 more source

Enhancing the Hydrolysis and Acyl Transfer Activity of Carboxylesterase DLFae4 by a Combinational Mutagenesis and In-Silico Method

Foods, 2023
In the present study, a feruloyl esterase DLFae4 identified in our previous research was modified by error-prone PCR and site-directed saturation mutation to enhance the catalytic efficiency and acyltransferase activity further. Five mutants with 6.9–118.
Longxiang Li, Liping Ding, Yuting Shao, Shengwei Sun, Mengxi Wang, Jiahui Xiang, Jingjie Zhou, Guojun Wu, Zhe Song, Zhihong Xin   +9 more
doaj   +1 more source