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Field-flow fractionation (FFF) is a family of instrumental techniques that separates and characterizes macromolecules, colloids, and particles (macromaterials) on an analytical scale (Colfen and Antonietti, 2000; Schimpf et al., 2000).
Schimpf, Martin E.
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Field‐flow fractionation for nanoparticle characterization
Journal of Separation Science, 2021AbstractThis review presents field‐flow fractionation: The elements of theory enable the link between the retention and the characteristics of the nanometer‐sized analytes to be highlighted. In particular, the nature of force and its way of being applied are discussed.
Gaëtane Lespes +1 more
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Micro-Thermal Field-Flow Fractionation
Collection of Czechoslovak Chemical Communications, 2002The effect of miniaturization of the separation channel on the performance of thermal field-flow fractionation (TFFF) is substantiated theoretically. The experiments carried out under carefully chosen experimental conditions proved the high performance of the separation of polymers within an extended range of molar masses from relatively low up to ...
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Sedimentation Field Flow Fractionation of Liposomes
Science, 1982Quantitative analyses of the particle size distributions of liposomes were performed in 30 to 60 minutes by exponential-field sedimentation field flow fractionation. This gentle new separation method exhibits great potential for the high-resolution fractionation and the size or molecular weight analysis of a wide variety of biological macromolecules ...
J J, Kirkland, W W, Yau, F C, Szoka
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Development of Electrical Field-Flow Fractionation
Analytical Chemistry, 2000Electrical field-flow fractionation (ElFFF) results for a series of polystyrene latex beads are presented. To first approximation, retention behavior can be related to conventional FFF theory, modified to account for a particle-wall repulsion effect.
, Tri, , Caldwell, , Beckett
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Sedimentation Field Flow Fractionation of DNA's
Science, 1984Sedimentation field flow fractionation (SFFF) is a method for purifying and providing mass or size distribution information on samples containing particulates or soluble macromolecules. Since SFFF separations are based on simple physical phenomena related to first principles, molecular weight (or particle sizes) can be determined without ...
L E, Schallinger +2 more
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Electrical Field-Flow Fractionation of Proteins
Science, 1972Protein separation has been achieved by electrical field-flow fractionation, a heretofore unrealized separation technique. Some advantages of this method relative to electrophoresis are the low voltage required, the lack of adverse heating and support effects, and the existence of the method as an elution technique.
K D, Caldwell +3 more
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Sedimentation Field Flow Fractionation: Applications
Science, 1982Sedimentation field flow fractionation is a powerful, new, high-resolution separation method for a wide variety of colloids, micelles, particulates, and soluble macromolecules of biological interest. Advances in instrumentation allow sedimentation field flow fractionation operation with rotor speeds up to 32,000 revolutions per minute (∼85,
J J, Kirkland, W W, Yau
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Field flow fractionation in biomedical analysis
Biomedical Chromatography, 1991AbstractSamples of biomedical interest which have been analysed by field‐flow fractionation techniques are surveyed. The list begins with whole cells and microorganisms, going through viruses, nucleic acids, cell fragments and organelles, down to proteins and their aggregates.
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Two-dimensional field-flow fractionation
Journal of Chromatography A, 1990Multidimensional field-flow fractionation (FFF) is described in two major forms: one in which different separative stages are coupled together and one in which two independent displacements, at least one of them FFF, are carried out in a generally planar channel structure.
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