Results 61 to 70 of about 35,116 (294)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Fast optimal control of asymmetric flow field flow fractionation processes
, 2022 S.207-213We present optimization problem for Asymmetric Flow Field Flow Fractionation, which is a widely used technique for segregation of two or more particles of submicron scale, according to their hydrodynamic radius.
Pinnau, R., Bayramov, N., Nagapetyan, T.
core +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Nanomaterials, 2018 The angular dependency of light scattering intensity from differently sized particles strongly influences the apparent particle size distribution, as determined by dynamic light scattering (DLS) methods. Manufactured nanomaterials have size distributions
Haruhisa Kato +2 more
doaj +1 more source
Wide-Range Size Fractionation of Graphene Oxide by Flow Field-Flow Fractionation
, 2022 Many interesting properties of 2D materials and their assembled structures are strongly dependent on the lateral size and size distribution of 2D materials.
Hayoung Yu (6415469) +10 more
core +1 more source
Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis
FEBS Open Bio, EarlyView.Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho +4 more
wiley +1 more source
, 2005 Biocompatible methods capable of rapid purification and fractionation of analytes from complex natural matrices are increasingly in demand, particularly at the forefront of biotechnological applications.
RESCHIGLIAN, PIERLUIGI +9 more
core
FEBS Open Bio, EarlyView.Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
Factors affecting particle retention in thermal field-flow fractionation
Bulletin of the Chemical Society of Ethiopia, 2002 In this paper, we report a range of factors which affect the retention of colloidal particles in thermal field-flow fractionation (ThFFF). These results are observed among different sizes of polystyrene (PS) latex particles suspended in both aqueous and ...
Paul M. Shiundu +2 more
doaj
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source