Results 101 to 110 of about 59,219 (244)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

SYMPATHIQUE: image-based tracking of symptoms and monitoring of pathogenesis to decompose quantitative disease resistance in the field

Plant Methods
Background Quantitative disease resistance (QR) is a complex, dynamic trait that is most reliably quantified in field-grown crops. Traditional disease assessments offer limited potential to disentangle the contributions of different components to overall
Jonas Anderegg, Radek Zenkl, Norbert Kirchgessner, Andreas Hund, Achim Walter, Bruce A. McDonald   +5 more
doaj   +1 more source

Characterization of Pre-Breeding Wheat (<i>Triticum aestivum</i> L.) Germplasm for Stripe Rust Resistance Using Field Phenotyping and Genotyping. [PDF]

Plants (Basel), 2023
Ul Islam B, Mir S, Dar MS, Khan GH, Shikari AB, Sofi NUR, Mohiddin F, Ahangar MA, Jehangir IA, Kumar S, Kumar S, Singh G, Wani SH.   +12 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Long-Term Alleviation of the Functional Phenotype in Chlorophyll-Deficient Wheat and Impact on Productivity: A Semi-Field Phenotyping Experiment. [PDF]

Plants (Basel), 2023
Colpo A, Demaria S, Baldisserotto C, Pancaldi S, Brestič M, Živčak M, Ferroni L.   +6 more
europepmc   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Outdoor Plant Segmentation With Deep Learning for High-Throughput Field Phenotyping on a Diverse Wheat Dataset. [PDF]

Front Plant Sci, 2021
Zenkl R, Timofte R, Kirchgessner N, Roth L, Hund A, Van Gool L, Walter A, Aasen H.   +7 more
europepmc   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Development of a high-throughput field phenotyping rover optimized for size-limited breeding fields as open-source hardware. [PDF]

Breed Sci, 2022
Kuroki K, Yan K, Iwata H, Shimizu KK, Tameshige T, Nasuda S, Guo W.   +6 more
europepmc   +1 more source