Results 81 to 90 of about 5,689,479 (302)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Accumulation efficiency of degradable matter during the early grain-filling period in rice [PDF]

, 2015
The dry weight of cellular contents in the whole rice plant (dWc/dt) is partitioned from the crop growth rate (dW/dt), and the resulting rate represents the accumulation efficiency of degradable matter (dWc/dW).
HAGIWARA, Motoyuki, INOUE, Naoto, KASAJIMA, Shinya, YANG, Zhongfa, KASUGA, Shigemitsu   +4 more
core  

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Design and Experimentation of a Roller-Type Precision Seed Metering Device for Rapeseed with Bezier Curve-Based Profiled Holes

Applied Sciences
To address the industry pain points of high seed breakage rate and uncontrollable miss-filling rate, multiple-filling rate in traditional rapeseed roller-type precision centralized seed metering devices—while breaking the adaptation limitation of ...
Huaili Pan, Hua Ji, Xinyu Hu, Yongqi Zhan, Guoliang Wei   +4 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Space-filling Techniques in Visualizing Output from Computer Based Economic Models [PDF]

One important factor concerning economic models is that frequently large amounts of data are produced. There is the research issue of how end-users (who may not be researchers or model developers) can be presented with this data so that maximum benefits ...
Wei Jiang, Richard Webber, Ric D Herbert
core  

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Filling vacancies: Identifying the most efficient recruitment channel [PDF]

This paper provides new results on the recruitment behaviour of firms by showing that recruitment channels clearly affect the probability of filling a vacancy. Using a French data, we estimate the effectiveness of different recruitment strategies, taking
Mareva Sabatier
core  

Modelling Afferent Nerve Responses to Bladder Filling [PDF]

, 2015
A sensation of fullness in the bladder is a regular experience, yet the mechanisms that act to generate this sensation remain poorly understood. This is an important issue because of the clinical problems that can result when this system does not ...
Holloway, Craig, Naire, Shailesh, Bayram, Saziye, Simitev, Radostin D., Daly, Donna M, Daly, Donna M., Simitev, Radostin D, Chakrabarti, Buddhapriya, Manhas, Varun, Dyson, Rosemary J, Clayton, Richard H., Shearer, Tom, Dyson, Rosemary J., Brook, Bindi, Argungu, Maryam, Clayton, Richard H   +15 more
core  

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source