Results 101 to 110 of about 4,004,190 (296)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Structure‐Performance Relationship of Electrospun PAN‐Based Nanofibrous Membranes for Air Filtration: Role of Surface Skewness and Packing Density

Macromolecular Materials and Engineering
Electrospun nanofibrous membranes are promising candidates for air decontamination because of their high surface‐to‐volume ratio, tunable pore structure, and low basis weight.
Erfan Sedighi Moshtaghin, Somaye Akbari, Farideh Golbabaei   +2 more
doaj   +1 more source

Modeling of Filtration Phenomenon in Hydrostatic Drives

Machines
Some users consider modern hydrostatic drives and controls to be unreliable and difficult to maintain. This view is often due to operational problems caused by issues with obtaining and then maintaining the appropriate cleanliness class of the working ...
Klaudiusz Klarecki, Dominik Rabsztyn, Piotr Czop   +2 more
doaj   +1 more source

Simulation d’une filtration liquide en canal poreux fermé à une extrémité avec prise en compte de la formation du dépôt [PDF]

, 2009
La formation d’un dépôt de filtration ainsi que les performances de filtration associées ont été étudiées par CFD en utilisant un maillage mobile.
CABASSUD, Corinne, SCHMITZ, Philippe, GUIGUI, Christelle, MENDRET, Julie   +3 more
core  

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Electret melt-blown nonwovens with charge stability for high-performance PM0.3 purification under extreme environmental conditions

e-Polymers
Electret technology is extensively utilized in fiber-based air filtration materials due to its ability to significantly enhance filtration efficiency (FE) without increasing the pressure drop.
Liu Chao, Shi Jiayan, Wang Zexing, Luo Fengxiang, Zhang Xing   +4 more
doaj   +1 more source

Filtration efficiency of nonofiber cloth

, 2016
In this bachelor thesis I deal with filtration efficiency of nanofiber cloth, which are determined by number of aerosol particles measured in front of the filter and behind the filter using APS. Aerosol particles are generated by CMAG.
Šimková, Kristýna
core  

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source