Results 181 to 190 of about 30,650 (267)

A General Workflow for Tangential Flow Filtration Perfusion Scale‐Up

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Tangential flow filtration (TFF) perfusion using hollow‐fiber modules offers substantial advantages over other mammalian cell retention technologies. Recently, increased awareness of Starling flow has helped to enhance filtration performance to similar, or even higher levels compared to alternative technologies.
Patrick Romann, Ken Lee, Venkatesh Natarajan, Daniel Ley, Claes Nymand Nilsson, David Garcia‐Munzer, Dominik Schieman, Mahbod Hajighasemi, Alex Zaytsev, Jason Friedman, Loic Chappuis, Tobias Habicher, Colin Jaques, Alex Cohen, Matthew Gagnon, Edward Chan, Daniel J. Karst, Philip Giller, Antony Sibilia, Andrew L. Zydney, Thomas K. Villiger   +20 more
wiley   +1 more source

Freshwater biodiversity in a rapidly changing Arctic: An expert horizon scan of key research questions. [PDF]

Ambio
Culp JM, Power M, Christoffersen KS, Goedkoop W, Kahilainen KK, Rautio M, Chaguaceda F, Hébert MP, Moedt SM, Antoniades D, Belle S, Bolnick D, Bouchard F, Brittain JE, Byström P, Chavarie L, Couture RM, Derry AM, Eloranta AP, Frainer A, Girard C, Grosbois G, Hessen DO, Hogg I, Jungblut AD, Lakka HK, Lam J, Lau DCP, Leblanc CA, Lento J, MacIntyre S, Marsh P, Martyniuk MAC, Milner A, Musetta-Lambert J, Mustonen T, Nowosad D, Saros JE, Schartau AK, Smol JP, Soininen J, Svenning MA, Tape KD, Wrona FJ.   +43 more
europepmc   +1 more source

Purification of Disassembled Capsid Protein by Hydroxyapatite Chromatography Enhances Virus‐Like Particle Recovery

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Virus‐like particles (VLPs) are a promising modality with extensive applications in prophylactic and therapeutic vaccine design. Human Papillomavirus (HPV) vaccines are one such example where VLP vaccine applications have led to the successful reduction in HPV‐associated diseases, such as cervical cancer.
Nicholas Samuelson, Nathaniel Parker, Shiyi Wang, Sara Daley, Aaron Cote, Michael A. Winters   +5 more
wiley   +1 more source

Effects of fish stocking and removal on plankton communities and trophic status in deep alpine-type lakes of the Five Lakes Valley (Tatra Mountains): Bottom-up and top-down regulation in the ecology of offshore animals. The lifetime passion of Z.Maciej Gliwicz. [PDF]

J Plankton Res
Ślusarczyk M, Brzeziński T, Dawidowicz P, Kapusta A, Zwijacz-Kozica T, Kuczyński T, Ochocka A, Kozłowski K.   +7 more
europepmc   +1 more source

Combinatorial Metabolic Engineering of Alkane Biosynthesis in the Osmotolerant Yeast Debaryomyces hansenii CBS 767

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Debaryomyces hansenii is a yeast with diverse potential applications like producing oleochemicals from biomass. Yet, combinatorial metabolic engineering in D. hansenii is limited because, like many nonconventional organisms, key genomic data and genetic elements are missing. Here, we report phenotypic characterization, genomic integration loci,
Zekun Li, Nilesh Kumar Sharma, Sarah Weintraub, Eric Young   +3 more
wiley   +1 more source

Physical and microbial root-zone factors underlying tomato wilt in long-term biofloc aquaponic systems. [PDF]

PLoS One
Rezaei S, Otto M, Wang Q, Arthur W, Shantharaj D, Sullivan K, Wells D, Potnis N, Higgins BT.   +8 more
europepmc   +1 more source

Whole‐Cell Production of Resolvin E2 and 17R‐Resolvin D5 by a Double‐Oxygenating 15S‐Lipoxygenase With 5S‐Lipoxygenase Activity From Pseudobdellovibrionaceae bacterium

Biotechnology and Bioengineering, EarlyView.
A recombinant whole‐cell platform expressing a bacterial 15S‐LOX exhibiting 5S‐LOX activity enabled the stereoselective production of RvE2 and 17R‐RvD5 from 18R‐HEPE and 17R‐HDHA, achieving final concentrations of 0.57 mM and 1.48 mM from 4.0 mM substrates, respectively, under optimized conditions.
Hee‐Gyeong Lee, Kyung‐Chul Shin, Deok‐Kun Oh   +2 more
wiley   +1 more source

Songbird population trajectories diverge under simulations of conifer encroachment versus removal in a sagebrush ecosystem. [PDF]

Ecol Appl
Zarri EC, Tack JD, Smith JT, Morford SL, Martin TE, Naugle DE.   +5 more
europepmc   +1 more source

Characterization of Immune Responses to RBD‐SD1 Nanoparticle Vaccines Derived From ACE2‐Capable HKU5‐CoV‐2, NeoCoV, and HKU5 Merbecoviruses

Biotechnology and Bioengineering, EarlyView.
SpyCatcher‐mi3 nanoparticles presenting HKU5, NeoCoV, and HKU5‐CoV‐2 RBD‐SD1 induced strong, cross‐reactive IgG responses in immunized mice. The surrogate virus neutralization test demonstrated strong activity in sera from homologous vaccination. ABSTRACT Recent discoveries of merbecoviruses such as HKU5, NeoCoV, and HKU5‐CoV‐2 that use angiotensin ...
Peter J. Halfmann, Jeong Soo Lee, Augustine Duffy, Tong Wang, Bingcheng Huang, James Z. Kurdi, Ajay Inampudi, Natasha Chen, Elizabeth Frimpong, Carmen Rai, Yoshihiro Kawaoka, Ravi S. Kane   +11 more
wiley   +1 more source

<i>Bacillus</i> spp. in Fish Farming: Enhancing Fish Performance, Health and Farming Practices. [PDF]

Aquac Nutr
Lu Y, Bambridge NB, Chen X, Feng Y.
europepmc   +1 more source