Results 201 to 210 of about 1,997,597 (283)

Isolation and Characterization of Random Citrate Synthase Variants That Increase Acetate Generation by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT A method to select Escherichia coli citrate synthase variants having reduced activity was developed based initially on the increased generation of acid, observed visually by prolonged color change in the pH indicator bromocresol purple. Five hundred fifty‐two potential variants were isolated and each were cultured on glucose as sole carbon ...
Jeffrey K. Dodelin, Ronnie L. Fulton, Abigail E. Rose, Mark A. Eiteman   +3 more
wiley   +1 more source

Diversity persists while function shifts: post-heatwave reorganization of reef fish communities in Baja California. [PDF]

PeerJ
Filz P, Beas-Luna R, Loiseau N, Lorda J, Freiwald J, Bauer J, Malpica-Cruz L.   +6 more
europepmc   +1 more source

Next‐Generation Glutamine Synthetase Expression Vector for Therapeutic Protein Manufacturing Using CHO Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The glutamine synthetase expression system is widely used for therapeutic protein biomanufacturing in Chinese hamster ovary (CHO) cells. Here, we present the design of an enhanced glutamine synthetase vector for generating CHOK1SV GS‐KO stable pools that produce recombinant proteins at multigram‐per‐liter levels. We first developed a synthetic
Yusuf B. Johari, Sophie J. Wensley, Malak A. Ammar, Avinash Srivastava, Changhavi Ragurajan, Jenish Patel, Natalie C. Elliott, Alice C. Hodgson‐Casson, Mariano Olivera Fedi, Michael Anbar, Peter M. O'Callaghan, Bernie Sweeney   +11 more
wiley   +1 more source

Water hyacinth vermicompost improves Nile tilapia (Oreochromis niloticus) growth and plankton dynamics in fertilized ponds: a comparative study with inorganic fertilizers. [PDF]

Sci Rep
Tolera G, Getahun A, Geremew A, Mengistou S.   +3 more
europepmc   +1 more source

Feasibility Assessment of a Single‐pH Connected Polishing Train for Monoclonal Antibody Purification Using Mildly Acidic Protein A Elution and an Acidic‐Window Anion Exchange Membrane

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody downstream processing often requires multiple pH adjustments between Protein A capture and polishing steps. These adjustments increase process complexity and make direct unit‐operation connection more difficult. We evaluated a connected post‐capture polishing block operated at approximately pH 5.0 using mild‐pH Protein A ...
Takaki Aiso, Hiroshi Muto, Kyota Suzuki, Yumiko Masuda, Hirofumi Kakihara, Masashi Sonoyama   +5 more
wiley   +1 more source

Continuous Capture of Recombinant Adeno‐Associated Virus Particles Using Twin‐Column CaptureSMB

Biotechnology and Bioengineering, EarlyView.
Continuous twin‐column CaptureSMB enables direct capture of secreted rAAV5 from minimally processed perfusion harvest, bypassing lysis, clarification, and concentration steps. Compared to batch capture, it achieves higher yield (+14.3%), greater productivity (+11.4%), and drastically reduced buffer use (−79.2%), while maintaining critical quality ...
Julia M. Müller, Daniela Tobler, Jules Bühler, Damian Hauri, Richard Plieninger, Sven Göbel, Efe Saygili, Yuki Higuchi, Ryosuke Takahashi, Sebastian Vogg, Thomas Müller‐Späth, Thomas K. Villiger   +11 more
wiley   +1 more source

Batch‐to‐Batch Optimization of pH Shifts in CHO Bioreactor Operations

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Culture pH is an important operating parameter in CHO cell cultures producing mAb since it directly affects growth and specific productivity. Mathematical models can be used to optimize the magnitude and timing of pH shifts provided that they are robust to model errors.
Ali Ghodba, Zahra Negahban, Anne Richelle, Chris McCready, Valerie Ward, Luis Ricardez‐Sandoval, Hector Budman   +6 more
wiley   +1 more source

Mitochondria‐endoplasmic reticulum organelle glue as maturation promoter for origination/dimensional dual‐cross cardiomyocytes

BMEMat, EarlyView.
Mitochondria‐endoplasmic reticulum contact sites (MERCS) are areas where the mitochondria and endoplasmic reticulum closely interact. In this study, we utilize synthetic organelle glues to artificially engineer MERCS for regulating cardiomyocyte development, through which the immature and chemo‐plasticity issues of undifferentiated cells are addressed.
Wei Tang, Hao Sun, Chenqi Duan, Jiajia Chang, Yuxing Wang, Ming Zhao, Huiyue Zhao, Shan Lu, Yang Yang, Da Huo   +9 more
wiley   +1 more source

Resolving the biome debate: ecological and palaeo‐data concur that the extensive tree‐limited grassy biomes of central and north‐western Madagascar are largely anthropogenic

Biological Reviews, EarlyView.
ABSTRACT For millennia, human activities have transformed ecosystems globally. Determining which landscapes are predominantly anthropogenic is crucial to conservation and habitat restoration. Madagascar, a critically‐threatened biodiversity hotspot, is a prominent example.
Grant S. Joseph, Sean Hixon, Colleen L. Seymour, Laurie R. Godfrey, Miguel Pedrono, Karen E. Samonds, Andrinajoro R. Rakotoarivelo, Brooke E. Crowley   +7 more
wiley   +1 more source

A Systemic Model for Understanding Business Interactions With Biodiversity and Ecosystems

Business Strategy and the Environment, EarlyView.
ABSTRACT Biodiversity loss and ecosystem degradation represent critical threats to human well‐being and economic resilience, challenging businesses to understand and manage their interdependence with natural systems. This study develops a systemic framework—the BioModel—that elucidates the reciprocal relationship between businesses, biodiversity, and ...
Lino Cinquini, Giacomo Pigatto, Andrea Tenucci   +2 more
wiley   +1 more source