ABSTRACT Adeno‐associated viral (AAV) vectors for gene therapy are becoming integral to modern medicine, providing therapeutic options for diseases once deemed incurable. Currently, viral vector purification is a critical bottleneck in the gene therapy industry, impacting product efficacy and safety as well as accessibility and cost to patients ...
Kelvin P. Idanwekhai +9 more
wiley +1 more source
Impact of biofilm support media on microbial dynamics, rocket (Eruca vesicaria subsp. sativa) growth, and antioxidative content in aquaponics. [PDF]
Ahmed AB +10 more
europepmc +1 more source
ABSTRACT Yeast sugar transporters have highly evolved for preferential glucose transport, a significant roadblock for utilizing non‐glucose sugars in renewable feedstocks such as lignocellulosic biomass. To enable simultaneous transport of multiple sugars, native hexose transporters were replaced by SWEET7p from Arabidopsis thaliana in engineered ...
Nurzhan Kuanyshev +6 more
wiley +1 more source
Signals From the Southern Edge: Demographic Effects of Ocean Warming on Two Cold-Adapted Seabird Species in the Gulf of Maine. [PDF]
Durham SE +3 more
europepmc +1 more source
Genetically Programmed Control of Overflow Metabolism
ABSTRACT Overflow metabolism is a highly undesirable phenomenon that occurs in virtually all cell factories. Although several schemes have been applied to avoid overflow metabolism, this problem remains far from being solved. In this study, a genetic sensor for detecting overflow metabolism was designed, and its substrate specificity and reversibility ...
Lærke M. Jensen +3 more
wiley +1 more source
An Antarctic ecosystem value index to quantify ecological value across trophic levels and over time. [PDF]
DuVivier AK +13 more
europepmc +1 more source
Raman Spectroscopy–Based Glucose Feed Control Automates Malic Acid Fermentation
Raman spectroscopy enables automated, closed‐loop glucose feeding for malic acid production with Ustilago trichophora. Online PLS regression of glucose and malic acid provides input for PID control to maintain 20 g/L glucose. The feed is dynamically adjusted and stopped in response to metabolic demand and product inhibition, demonstrating practical ...
Luca Antonia Grebe +8 more
wiley +1 more source
Valorization of fish processing by-products through combined enzymatic and microbial hydrolysis: nitrogen recovery and fertilizer efficiency in wheat. [PDF]
Solis T +5 more
europepmc +1 more source
Optimizing Pichia Pastoris Cell‐Free Protein Synthesis to Improve Economics
ABSTRACT Cell‐free protein synthesis (CFPS) is a powerful and versatile platform that supports a wide range of applications, from fundamental studies of the genetic code to scalable and rapid protein production. The recently developed Pichia pastoris CFPS combines advantages of both prokaryotic and eukaryotic systems, including a rapid growth rate ...
Rui Wu +4 more
wiley +1 more source

