Results 161 to 170 of about 1,910,950 (190)

GlycoMAC: A Multiscale Metabolic‐Glycosylation Framework for Predicting Glycosylation Across Conditions in Mammalian Cell Cultures

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Antibody productivity and glycosylation quality in CHO cell cultures emerge from a dynamically evolving metabolic environment, yet existing models often work in isolation or at a single scale. Here, we present a multiscale mechanistic framework linking molecular, cellular, and macroscopic scales to predict how inputs shape bioprocess ...
Yuming Zeng, Sarah W. Harcum, Jinxiang Pei, Wei Xie   +3 more
wiley   +1 more source

Isolation and Characterization of Random Citrate Synthase Variants That Increase Acetate Generation by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT A method to select Escherichia coli citrate synthase variants having reduced activity was developed based initially on the increased generation of acid, observed visually by prolonged color change in the pH indicator bromocresol purple. Five hundred fifty‐two potential variants were isolated and each were cultured on glucose as sole carbon ...
Jeffrey K. Dodelin, Ronnie L. Fulton, Abigail E. Rose, Mark A. Eiteman   +3 more
wiley   +1 more source

Next‐Generation Glutamine Synthetase Expression Vector for Therapeutic Protein Manufacturing Using CHO Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The glutamine synthetase expression system is widely used for therapeutic protein biomanufacturing in Chinese hamster ovary (CHO) cells. Here, we present the design of an enhanced glutamine synthetase vector for generating CHOK1SV GS‐KO stable pools that produce recombinant proteins at multigram‐per‐liter levels. We first developed a synthetic
Yusuf B. Johari, Sophie J. Wensley, Malak A. Ammar, Avinash Srivastava, Changhavi Ragurajan, Jenish Patel, Natalie C. Elliott, Alice C. Hodgson‐Casson, Mariano Olivera Fedi, Michael Anbar, Peter M. O'Callaghan, Bernie Sweeney   +11 more
wiley   +1 more source

Feasibility Assessment of a Single‐pH Connected Polishing Train for Monoclonal Antibody Purification Using Mildly Acidic Protein A Elution and an Acidic‐Window Anion Exchange Membrane

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody downstream processing often requires multiple pH adjustments between Protein A capture and polishing steps. These adjustments increase process complexity and make direct unit‐operation connection more difficult. We evaluated a connected post‐capture polishing block operated at approximately pH 5.0 using mild‐pH Protein A ...
Takaki Aiso, Hiroshi Muto, Kyota Suzuki, Yumiko Masuda, Hirofumi Kakihara, Masashi Sonoyama   +5 more
wiley   +1 more source

Mitochondria‐endoplasmic reticulum organelle glue as maturation promoter for origination/dimensional dual‐cross cardiomyocytes

BMEMat, EarlyView.
Mitochondria‐endoplasmic reticulum contact sites (MERCS) are areas where the mitochondria and endoplasmic reticulum closely interact. In this study, we utilize synthetic organelle glues to artificially engineer MERCS for regulating cardiomyocyte development, through which the immature and chemo‐plasticity issues of undifferentiated cells are addressed.
Wei Tang, Hao Sun, Chenqi Duan, Jiajia Chang, Yuxing Wang, Ming Zhao, Huiyue Zhao, Shan Lu, Yang Yang, Da Huo   +9 more
wiley   +1 more source

Evidence for adaptive explanations of semelparity in animals

Biological Reviews, EarlyView.
ABSTRACT Semelparity, the reproductive strategy of reproducing once, is widespread but uncommon in animals. Classes of models to explain the evolution of semelparity are based either on age structure and mortality schedules – demographic models in which high post‐reproductive mortality risk favours high reproductive effort and semelparity results from ...
Diana O. Fisher, Roberto Salguero‐Gómez   +1 more
wiley   +1 more source

Carrion ecology: concepts, interdisciplinary synthesis, and perspectives

Biological Reviews, EarlyView.
ABSTRACT Carrion is a ubiquitous resource in both terrestrial and aquatic ecosystems, yet it has long been overlooked in ecological research. Over the past two decades, studies on carrion and the many organisms that exploit it have flourished, revealing not only wide‐ranging ecological functions but also significance far beyond ecology.
Marcos Moleón, José A. Sánchez‐Zapata, Travis L. DeVault, Wayne M. Getz, Zebensui Morales‐Reyes, Daniel Redondo‐Gómez, Lara Naves‐Alegre, Solange A. Batista‐Nunes, Esther Sebastián‐González, Juan M. Pérez‐García, Kelly A. Waters, Thijs van Overveld, Craig Smith, Nuria Selva, Thomas A. Schlacher, Graeme Ruxton, Jesús Rodríguez, Martina‐M. Quaggiotto, Paula L. Perrig, Norman Owen‐Smith, Thomas M. Newsome, Isabel Navas, Yoshihiro Nakashima, Ana Mateos, Carlos Martínez‐Carrasco, Daniel Martín‐Vega, Berta Martín‐López, Antoni Margalida, Sergio A. Lambertucci, Adam Kane, Kevin Healy, Akane Hashizume, Antonio J. García‐Fernández, José A. Donázar, M. Eric Benbow, James C. Beasley, Philip S. Barton, Kathleen A. Alexander, Eneko Arrondo   +38 more
wiley   +1 more source

Resolving the biome debate: ecological and palaeo‐data concur that the extensive tree‐limited grassy biomes of central and north‐western Madagascar are largely anthropogenic

Biological Reviews, EarlyView.
ABSTRACT For millennia, human activities have transformed ecosystems globally. Determining which landscapes are predominantly anthropogenic is crucial to conservation and habitat restoration. Madagascar, a critically‐threatened biodiversity hotspot, is a prominent example.
Grant S. Joseph, Sean Hixon, Colleen L. Seymour, Laurie R. Godfrey, Miguel Pedrono, Karen E. Samonds, Andrinajoro R. Rakotoarivelo, Brooke E. Crowley   +7 more
wiley   +1 more source

Corporate Decarbonization via Technology and Management

Business Strategy and the Environment, EarlyView.
ABSTRACT This study provides a comprehensive overview of key findings on decarbonization, advanced technologies, and management strategies, highlighting emerging themes shaping the field. Advanced technologies enhance carbon reduction through efficiency, real‐time monitoring, and optimizing resource optimization.
Heidy Montero‐Teran, Rudolf R. Sinkovics, Olli Kuivalainen   +2 more
wiley   +1 more source

Shaping Energy Transitions: Sectoral Demand, Climate Risk Exposure, and Renewable Pathways in Sub‐Saharan Africa

Business Strategy and the Environment, EarlyView.
ABSTRACT Energy is a fundamental driver of economic growth, shaping productivity, industrialization, and long‐term economic resilience. In sub‐Saharan Africa (SSA), where energy access remains uneven and infrastructure is underdeveloped, understanding sector‐specific energy demand is essential for designing sustainable energy strategies.
Michael Appiah, Emmanuel Baffour Gyau, Genanew Bekele Worku, Simplice A. Asongu   +3 more
wiley   +1 more source