Results 231 to 240 of about 84,111 (254)

Electron Microscopy of Fission Yeast

Cold Spring Harbor Protocols, 2017
Electron microscopy (EM) can provide images of cells with a spatial resolution that significantly surpasses that available from light microscopy (LM), even with modern methods that give LM “super resolution.” However, EM resolution comes with costs in time spent with sample preparation, expense of instrumentation, and concerns regarding sample ...
J Richard, McIntosh, Mary K, Morphew, Thomas H, Giddings   +2 more
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Tetrad Dissection in Fission Yeast

2018
Tetrad dissection is a powerful tool in yeast genetics that allows the analysis of products of a single meiosis. With just a few tetrads, it is possible to determine linkage, identify unique phenotypes associated with double mutants, or assess specific meiotic defects. Strains are crossed on nitrogen-limiting medium for 3 days.
Wilber, Escorcia, Susan L, Forsburg
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Transcriptional silencing in fission yeast

Journal of Cellular Physiology, 2000
In eukaryotes, epigenetic events govern diverse processes, ranging from gene expression to other aspects of global chromosome architecture essential for preserving the integrity of the genome. Transcriptional silencing at the mating-type locus, centromeres, and telomeres of the fission yeast is regulated by epigenetic mechanisms.
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Chromatin Immunoprecipitation in Fission Yeast

2011
A tremendous amount of information regarding the nature and regulation of heterochromatin has emerged in the past 10 years. This rapid progress is largely due to the development of techniques such as chromatin immunoprecipitation or "ChIP," which allow analysis of chromatin structure. Further technological advances such as microarray analysis and, more
Thomas A, Volpe, Jessica, Demaio
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Centromeric chromatin in fission yeast

Frontiers in Bioscience, 2008
A fundamental requirement for life is the ability of cells to divide properly and to pass on to their daughters a full complement of genetic material. The centromere of the chromosome is essential for this process, as it provides the DNA sequences on which the kinetochore (the proteinaceous structure that links centromeric DNA to the spindle ...
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Cyclin in fission yeast

Cell, 1988
Genetic studies of the fission yeast cell cycle have identified the cdc2 gene as a critical regulator of M phase (Nurse and Thuriaux, 1980). The gene encodes a protein kinase (Simanis and Nurse, 1986) that is homologous to the product of the CDC28 cell cycle "start" gene of budding yeast (Beach et al., 1982).
Mark Solomon, Robert Booher, Marc Kirschner, David Beach   +3 more
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Molecular control of fission yeast cytokinesis

Seminars in Cell & Developmental Biology, 2016
Cytokinesis gives rise to two independent daughter cells at the end of the cell division cycle. The fission yeast Schizosaccharomyces pombe has emerged as one of the most powerful systems to understand how cytokinesis is controlled molecularly. Like in most eukaryotes, fission yeast cytokinesis depends on an acto-myosin based contractile ring that ...
Sergio A, Rincon, Anne, Paoletti
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Random Spore Analysis in Fission Yeast

2018
Random spore analysis (RSA) is a tool that allows for the screening of a large number of meiotic products. It requires only a limited effort, and is often the method of choice for constructing strains with unambiguous genotypes. It is also useful to identify the frequency of rare events. Strains are crossed on a nitrogen-limiting medium for three days.
Wilber, Escorcia, Susan L, Forsburg
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Cytokinesis in fission yeast Schizosaccharomyces pombe

1997
Publisher Summary This chapter discusses the cytokinesis in fission yeast Schizosaccharomyces pombe. The process of cytokinesis in animal cells has been the subject of a large number of ingenious embryological experiments. While these experiments have revealed a great deal at a mechanistic level about what factors influence cleavage furrow formation,
M K, Balasubramanian, D, McCollum, K L, Gould   +2 more
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Ribosome Numbers in a Fission Yeast

Nature, 1965
THERE is a need for a more confined and precise use of the word ribosome. It would clarify molecular biology if the word were reserved for particles in the cell which measure 100 A–300 A in diameter, are composed of RNA and protein, and have been shown to be active as sites for the synthesis of proteins.
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