Results 111 to 120 of about 59,495,041 (290)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Development of fit-for-purpose project management

, 2021
The problem defined in this research is that the concept “fit-for-purpose” does not have a widely accepted definition in the research field of PM (project management).
MA, TIANLIN (author)
core  

Exodontia curriculum evaluation: fit for purpose teaching and learning strategies

Frontiers in Education
IntroductionCurriculum review is crucial for ensuring health professions education programs remain responsive and relevant. Teaching and learning (T&L) strategies facilitate knowledge acquisition, with traditional methods being supplemented by ...
Nashreen Behardien, Simone Titus, Simone Titus, Nicolette V. Roman   +3 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Not fit for purpose

, 2012
Bite: Artists Making Prints is a British printmaking survey show selected by Francis Tinsley, Anne Desmet, Tom Hammick, Jane Hindley, and Lance Trevellyan.
Mumberson, S.
core  

Bayesian diagnostic accuracy estimation of milk enzyme-linked immunosorbent assay, blood polymerase chain reaction, and peripheral blood lymphocyte count tests to determine bovine leukosis virus status in dairy cows

Journal of Dairy Science
: We assessed the diagnostic accuracy of an adapted antibody ELISA (ELISA-Ab) test, originally designed for bulk milk samples but applied on individual DHI-collected milk samples, to identify the bovine leukosis virus infection status of individual cows.
K.G. Solano-Suarez, J.C. Arango-Sabogal, J.P. Roy, E. Molgat, C. Bédard, C.A. Gagnon, S. Buczinski, S. Dufour   +7 more
doaj   +1 more source