Results 61 to 70 of about 93,969 (292)

Optimized fixation of actin filaments for improved indirect immunofluorescence staining of rickettsiae

open access: yesBMC Research Notes, 2019
Objective The objective was to investigate fixative solutions: 3.7% formaldehyde, 4% paraformaldehyde, 4% paraformaldehyde in the cytoskeletal buffer and 4% paraformaldehyde in PHEM buffer (containing PIPES, HEPES, EGTA and MgCl2), applicable for ...
Monika Danchenko   +3 more
doaj   +1 more source

Rupestonic Acid of Artemisia Rupestris L. Extract Treats Pulmonary Fibrosis in COPD by Targeting TGF‐β1

open access: yesAdvanced Science, EarlyView.
RA of EEAR inhibits TGF‐β1 ubiquitination and changes conformation by target binding TGF‐β1, regulating TGF‐β1/Smad2/3 signaling pathway. Thus it down‐regulated downstream protein expression, inhibited EMT and collagen deposition of ECM, in order to EEAR preventing PF in COPD.
Lingfeng Peng   +6 more
wiley   +1 more source

Structural and Functional Characterization of EXPO‐Derived Extracellular Vesicles in Plants

open access: yesAdvanced Science, EarlyView.
In this study, 3D electron tomography (ET), cryo‐ET, and immunogold transmission electron microscopy (TEM) are employed to characterize plant extracellular vesicles (EVs) under physiological conditions. EVs are classified into three distinct categories according to their size, content, and molecular‐marker profiles. Furthermore, Exo70E2‐positive medium
Jiayang Gao   +12 more
wiley   +1 more source

Evaluation of fixative solutions for ultrastructural analysis of brown spider Loxosceles intermedia (araneae: sicariidae) tissues

open access: yesBrazilian Journal of Biology
In view of the widely varying compositions of fixative solutions used for studying spiders, five different fixative formulas were tested for fixing male brown-spider (Loxosceles intermedia) gonad tissues.
C. L. S. Costa-Ayub   +2 more
doaj   +1 more source

Distribution of alkaline and acid phosphatases in the duodenal wall of native sheep by using different fixatives [PDF]

open access: yesIraqi Journal of Veterinary Sciences, 2010
Ten duodeni of adult ram were fixed in chilled acetone, 80% ethyl alcohol, formol- alcohol solution, alcoholic bouinssolution and neutral buffered formalin solution.
N. S. Ahmed
doaj  

Name of Fixative [PDF]

open access: hybrid, 2020
National Cancer Institute
openalex   +1 more source

Nanoscale Mapping of the Subcellular Glycosylation Landscape

open access: yesAdvanced Science, EarlyView.
Using multiplexed super‐resolution imaging with fluorophore‐labeled lectins, this study reports intracellular glycosylation at the nanoscale across organelles and synaptic specializations. Extending glycan analysis beyond the cell surface, Glyco‐STORM reveals distinct glycosylation nanodomains in the ER, Golgi, lysosomes, and synaptic sites.
Helene Gregoria Schroeter   +4 more
wiley   +1 more source

Use of saturated sodium chloride solution as a tissue fixative [PDF]

open access: yesIraqi Journal of Veterinary Sciences, 2010
The present study was carried out to examine the capability of saturated sodium chloride solution as a fixative agent instead of formalin which is regarded as a carcinogenic material.
A. Al-Saraj
doaj  

YKT6 Promotes Bladder Cancer Progression by Stabilizing β‐catenin Through USP7‐Mediated Deubiquitination

open access: yesAdvanced Science, EarlyView.
The SNARE protein YKT6 is upregulated in bladder cancer (BLCA), correlating with poor prognosis. YKT6 promotes tumor proliferation and metastasis by activating Wnt/β‐catenin signaling. It recruits Ubiquitin‐Specific Peptidase 7 (USP7) to deubiquitinate and stabilize β‐catenin, enhancing its nuclear accumulation and driving oncogenic gene expression ...
Sheng Tu   +16 more
wiley   +1 more source

DNA Extraction from Bronchial Aspirates for Molecular Cytology: Which Method to Take?

open access: yesAnalytical Cellular Pathology, 2003
Objective: To date, there are only few systematic reports on the quality of DNA extracted from routine diagnostic cytologic specimens. It was the aim of the present study to evaluate the ability of 50% ethanol/2% carbowax (Saccomanno fixative) to ...
Hans Jürgen Grote   +3 more
doaj   +1 more source

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