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The fish pathogen Flavobacterium columnare represents four distinct species: Flavobacterium columnare, Flavobacterium covae sp. nov., Flavobacterium davisii sp. nov. and Flavobacterium oreochromis sp. nov., and emended description of Flavobacterium columnare

Systematic and Applied Microbiology, 2022
Flavobacterium columnare is the causative agent of columnaris disease in freshwater fish and four discrete genetic groups exist within the species, suggesting that the species designation requires revision. The present study determined the taxonomic status of the four genetic groups of F.
Benjamin R, LaFrentz   +12 more
openaire   +2 more sources

Use of suppressive subtractive hybridization to identify Flavobacterium columnare DNA sequences not shared with Flavobacterium johnsoniae [PDF]

open access: yesLetters in Applied Microbiology, 2008
To identify specific sequences in the fish pathogen Flavobacterium columnare not shared by Flavobacterium johnsoniae.Suppressive subtractive hybridization (SSH) was used to selectively amplify and clone F. columnare-specific sequences. A highly virulent strain of F. columnare was used as tester and the type strain of F.
C R Arias
exaly   +3 more sources

Ungeremine and Its Hemisynthesized Analogues as Bactericides against Flavobacterium columnare

Journal of Agricultural and Food Chemistry, 2013
The Gram-negative bacterium Flavobacterium columnare is the cause of columnaris disease, which can occur in channel catfish ( Ictalurus punctatus ). In a previous study, the betaine-type alkaloid ungeremine, 1, obtained from Pancratium maritimum L. was found to have strong antibacterial activity against F. columnare.
Kevin K Schrader   +2 more
exaly   +4 more sources

Laboratory Maintenance of Flavobacterium psychrophilum and Flavobacterium columnare

Current Protocols in Microbiology, 2007
AbstractBacteria in the genus Flavobacterium are highly heterogeneous and are comprised of pathogenic and non‐pathogenic species. They are found in a wide variety of environments that include fresh and marine water, soil, and ocean sediments. This unit focuses on laboratory maintenance of two important fish pathogens within this genus.
Kenneth D, Cain, Benjamin R, Lafrentz
openaire   +2 more sources

Genetic fingerprinting of Flavobacterium columnare isolates from cultured fish

Journal of Applied Microbiology, 2004
To evaluate the intraspecific diversity of the fish pathogen Flavobacterium columnare.Genetic variability among Fl. columnare isolates was characterized using restriction fragment length polymorphism analysis of the 16S rDNA gene, intergenic spacer region (ISR) sequencing, and amplified fragment length polymorphism (AFLP) fingerprinting.
C A Shoemaker, P H Klesius, P H Klesius
exaly   +3 more sources

Identification of an iron acquisition machinery in Flavobacterium columnare

Diseases of Aquatic Organisms, 2013
Flavobacterium columnare, a fastidious Gram-negative pathogen and the causative agent of columnaris disease, is one of the most harmful pathogens in the freshwater fish-farming industry. Nevertheless the virulence mechanisms of F. columnare are not well understood.
Lingyu, Guan   +4 more
openaire   +2 more sources

Multiplex PCR for genotypingFlavobacterium columnare

Journal of Fish Diseases, 2019
AbstractRecent research has identified four distinct genetic groups among isolates ofFlavobacterium columnarethrough multilocus phylogenetic analyses; however, there are no quick methods to determine the genotype of an isolate. The objective of this research was to develop a multiplex PCR to rapidly genotypeF. columnareto genetic group.
Benjamin R. LaFrentz   +2 more
openaire   +2 more sources

Growth of Flavobacterium columnare Genomovars in the Presence or Lack of Supplemental Cations

Current Microbiology, 2021
Flavobacterium columnare is a problematic pathogen for the aquaculture industry where isolates are classified by genomovars. Suspended growth in a low nutrient media, like tryptone yeast extract salts, is a common method used for laboratory study.
Jason P. Evenhuis   +2 more
openaire   +2 more sources

Flavobacterium columnare colony types: Connection to adhesion and virulence?

Microbial Pathogenesis, 2009
Four different colony morphologies were produced by Flavobacterium columnare strains on Shieh agar plate cultures: rhizoid and flat (type 1), non-rhizoid and hard (type 2), round and soft (type 3), and irregularly shaped and soft (type 4). Colonies produced on AO agar differed from these to some extent.
Heidi M T, Kunttu   +3 more
openaire   +2 more sources

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