Results 51 to 60 of about 54,107 (281)

Leucine‐rich glioma inactivated 1 (LGI1) is a ganglioside‐binding protein

FEBS Letters, EarlyView.
Neuronal hyperexcitability associated with a decrease/absence of the extracellular protein LGI1 has been suggested to be primarily due to the downregulation of Kv1 channel expression. The molecular mechanisms underlying this decrease have not yet been elucidated.
Kévin Debreux, Christian Lévêque, Fodil Azzaz, Marion Sangiardi, Sarosh R Irani, Michael Seagar, Jacques Fantini, Oussama El Far   +7 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Design and Analysis of Power-function-sine Flexure Hinges

Jixie chuandong, 2023
In this study, a new type of power-function-sine flexure hinge is proposed. The calculation formula of compliance and rotation accuracy of the flexure hinge is derived by using Castigliano's second theorem.
Li Jiabiao, Zhao Yang, Wu Qingwen, Mu Deqiang, Liu Changru, Hu Jinxin   +5 more
doaj  

Analysis and Design of a Spherical Micromechanism With Flexure Hinges

Journal of Mechanical Design, 2009
Abstract The article describes the design of a robotic wrist able to perform spherical motions: Its mechanical architecture is based on parallel kinematics and is suitable to be realized at the mini- or microscale by means of flexible joints.
CALLEGARI M, CAMMARATA, ALESSANDRO, GABRIELLI A, RUGGIU M, SINATRA, Rosario   +4 more
openaire   +2 more sources

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Theoretical Stiffness Modeling and Application Research of a Novel Stacked Flexure Hinge

Aerospace, 2023
This study investigates and designs a novel stacked hinge with low stiffness, large rotation angle, high strength, and length-adaptive functionality. Firstly, based on the large deformation theory of cantilever beams and relevant theories of leaf springs,
Yonghong Zhang, Chengmin Wang, Shuangquan Tang, You Jiang, Hong Chen, Wenjie Ge   +5 more
doaj   +1 more source

Anisotropic flexure hinges: Manufacturing and mechanical characterization forapplication in pressure-actuated morphing structures

, 2023
Shape-morphing structures based on pressure actuation promise efficiency gains and performance improvements in aeronautics, but require complex three-dimensional geometries.
Sennewald, Cornelia, Vorhof, Michael, Hühne, Christian, Meyer, Patrick, Koord, Josef, Cherif, Chokri   +5 more
core   +2 more sources

Design and testing of multi-material flexure hinges for Fused Filament Fabrication [PDF]

, 2023
[EN] Flexure hinges are non-assemble flexible joints that allow the relative rotation of two adjacent rigid parts through bending. Traditionally thermoplastic material hinges are made through injection moulding.
Ermolai, Vasile, Nagît, Gheorghe, Sover, Alexandru   +2 more
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source