Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A Dataset of Vertical Carbon Fluxes from a Georgia Tidal Salt Marsh from 2014 to 2024. [PDF]
Hawman PA, Mishra DR.
europepmc +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Development of an electrochemiluminescence-based bridging assay to detect antibodies against a PTH inverse agonist in human plasma. [PDF]
Nduwumwami AJ +5 more
europepmc +1 more source
Hardware-aware knowledge-distilled CNN for real-time classification of plaque-associated angiographic findings on FPGA. [PDF]
Pravalika N, Afthab J, Rajamani V.
europepmc +1 more source
Size estimation of mixed free-floating sub-resolution spherical scatterers utilizing adaptive scanning optical coherence tomography. [PDF]
Tian Y, Izatt JA, Kuo A, Mcnabb RP.
europepmc +1 more source
MOF-Derived Cu-TiO<sub>2</sub>/Alginate Micromotors for Buoyancy-Regulated 3D Motion. [PDF]
Zou W +7 more
europepmc +1 more source
Benchmarking of Multi-Modal Partial Discharge Sensors and a Cross-Modal Corroboration Framework for Noise Discrimination in Air-Insulated Medium-Voltage Metal-Clad Switchgear. [PDF]
Shahsavarian T +4 more
europepmc +1 more source
The dynamic response of Pine Island Glacier to two decades of intermittent ice shelf regrounding. [PDF]
Surawy-Stepney T +5 more
europepmc +1 more source
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