Results 181 to 190 of about 1,828,109 (317)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Scaling Aspects of Lyari River Flow Routing [PDF]

open access: yes
In this communication we utilize an improved version for model proposed by Manning for the waste flow via an open channel Lyari. We have computed the status of discharges, storages and depth for all locations of the Lyari waste flow using this proposed ...
Asif Mansoor, Rana Khalid Naeem
core  

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

Analysis of Flow Characteristics of Flaring Pier Combined with Underflow Energy Dissipation Based on Three-Dimensional Numerical Simulation

open access: yesRenmin Zhujiang
Ensuring safe flood discharge and efficient energy dissipation under high head and large unit discharge is a critical challenge in high dam engineering. The combination of flaring piers and underflow energy dissipation provides an effective solution.
CHEN Biao, LIU Tao
doaj  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

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