Results 201 to 210 of about 2,292,052 (266)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Evaluation of Fluid Behaviors in a Pushbutton-Activated Microfluidic Device for User-Independent Flow Control. [PDF]

Micromachines (Basel)
Han DH, Lee G, Oh U, Choi Y, Park JK.
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Coil embolization of a celiac artery pseudoaneurysm under balloon-assisted flow control: A case report. [PDF]

Radiol Case Rep
Sekino H, Ishii S, Ishigame T, Ando T, Kawamoto N, Endo Y, Kuroiwa D, Ito H.   +7 more
europepmc   +1 more source

3D printed microfluidic valve on PCB for flow control applications using liquid metal. [PDF]

Biomed Microdevices
Hamza A, Navale A, Song Q, Bhagwat S, Kotz-Helmer F, Pezeshkpour P, Rapp BE.   +6 more
europepmc   +1 more source

Structural optimization of multistage depressurization sleeve of axial flow control valve based on Stacking integrated learning. [PDF]

Sci Rep
Li S, Deng G, Hu Y, Yu M, Ma T.
europepmc   +1 more source

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Flow control scheduling

Microprocessors and Microsystems, 2003
Abstract We address a problem associated with credit flow control (FC) schemes for buffered switches, namely, the issue of FC bandwidth and FC optimization, i.e. how many and which credits to return per packet cycle. Using simulations, we show that, under the assumption of bursty traffic with uniform or nonuniform destinations, the number of credits ...
Ferdinand Gramsamer, Mitchell Gusat, Ronald P. Luijten   +2 more
openaire   +1 more source

Flow control with hydrogels

Advanced Drug Delivery Reviews, 2004
With the advent of the genomic revolution and the sequencing of the human genome complete, the majority of pharmaceuticals under development are proteins. Consequently, new techniques to more effectively administer these new protein therapeutics need to be developed.
David T, Eddington, David J, Beebe
openaire   +2 more sources