Results 181 to 190 of about 1,032,869 (292)

Enrichment of Neural Crest Cells by Antibody Labeling and Flow Cytometry for Single-Cell Transcriptomics in a Lizard. [PDF]

Evol Dev
Pranter R, Patthey C, Feiner N.
europepmc   +1 more source

Shellac‐Mediated Assembly of Nanoparticles for mRNA Delivery

Advanced Healthcare Materials, EarlyView.
A shellac‐mediated nanoparticle assembly strategy is presented, involving the complexation of mRNA and low‐molecular‐weight cationic molecules (e.g., polyethyleneimine, chitosan, lipids, and poly‐l‐lysine), followed by capping with shellac. This nanoparticle platform enables mRNA transfection following intravenous injection and facilitates gene editing
Meizhang Lu, Jingqu Chen, Zhixing Lin, Wanjun Xu, Tianzheng Wang, Shiyao Li, Die Yang, Frank Caruso   +7 more
wiley   +1 more source

Protocol for multi-organ isolation of murine leukocytes for flow cytometry. [PDF]

STAR Protoc
Layug PJ, Marcinow A, Sontag D, Mendelson AA, Arsenio J.   +4 more
europepmc   +1 more source

Plant‐Produced Viral Nanoparticles Decorated with Nanobodies Against HER2 Improve Retention and Recruitment of Immune Cells in Solid Tumors

Advanced Healthcare Materials, EarlyView.
Potato virus X is a filamentous RNA plant virus that can be engineered into a molecular tool for cancer therapy. We produced genetically‐encoded virus‐derived nanoparticles decorated with nanobodies targeting cancer cell receptors, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2).
Enrique Lozano‐Sanchez, Miguel A. Moreno‐Gonzalez, Fernando Merwaiss, Nicole F. Steinmetz, José‐Antonio Daròs   +4 more
wiley   +1 more source

Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamples. [PDF]

Cell Rep Methods
Bai Y, Wang Y, Fu Y, Guo Z, Liang Z, Yang L, Ribaudo J, Liu D, Li Y, Zhang T, Xue L, Yang J, Liu H, Li X, Zhang J.   +14 more
europepmc   +1 more source

Flow cytometry

British Journal of Anaesthesia, 1997
H F Galley, N R Webster
openaire   +1 more source

A Superhydrophobic 3D Cell Culture System Reveals the Mechanobiological Role of Cancer‐Associated Fibroblasts in Prostate Cancer Metastasis

Advanced Healthcare Materials, EarlyView.
A superhydrophobic 3D cell culture platform enables rapid and consistent formation of heterotypic tumor–stroma clusters, and reveals how physiological shear conditions influence metastatic signaling. Mechanical stimulation of cancer‐associated fibroblasts promotes sustained cytokine secretion and survival‐promoting pathways, showing how multicellular ...
Alexandria T. Carter, Abigail R. Fabiano, Ehsan Aalaei, Jason Deng, Dakota E. Rostant, Michael R. King   +5 more
wiley   +1 more source

AutoFlow: an interactive Shiny app for supervised and unsupervised flow cytometry analysis. [PDF]

Bioinformatics
Woods FER, Leonard E, Ebbels T, Cairns J, David R.   +4 more
europepmc   +1 more source

3D Soft Hydrogels Induce Human Mesenchymal Stem Cells “Deep” Quiescence

Advanced Healthcare Materials, EarlyView.
Three‐dimensional soft hydrogels mimicking the bone marrow niche induce deep quiescence in human mesenchymal stem cells. Unlike 2D culture, 3D matrices halt proliferation, regulate cell‐cycle and quiescence markers, and downregulate mTORC1 signaling, preserving stem cell phenotype and therapeutic potential ex vivo.
David Boaventura Gomes, Timo Rademakers, Ana Filipa Henriques Lourenço, Andrea Calore, Clarissa Tomasina, Denis van Beurden, Jinjie Venema, Bryan Chömpff, Hong Liu, Nicole Bouvy, Sandra Camarero‐Espinosa, Lorenzo Moroni   +11 more
wiley   +1 more source

Fluorescent TAPY-bodipy dyads as tools for imaging fungal mitochondria by confocal microscopy and flow cytometry. [PDF]

Anal Bioanal Chem
Neto JC, de Llanos R, Galindo F.
europepmc   +1 more source