Results 271 to 280 of about 5,219,938 (357)

Isolation Defines Identity: Functional Consequences of Extracellular Vesicle Purification Strategies

Advanced Healthcare Materials, EarlyView.
Four extracellular vesicle purification strategies are compared using ovarian‐cancer ascites and ES‐2 cell supernatants. A novel workflow links purification to function by combining particle‐normalized proteomics with matched cell‐free and cell‐based assays.
Christian Preußer, Dolores J. Salander, Witold Szymanski, Johannes Graumann, Yukai Wang, Kai Zhao, Jörg W. Bartsch, María Gómez‐Serrano, Daniel Bachurski, Silke Reinartz, Elke von Pogge von Strandmann   +10 more
wiley   +1 more source

Standardization of Measurable Residual Disease in Acute Myeloid Leukemia by Flow Cytometry: A Multicenter Study. [PDF]

EJHaem
Ikoma-Colturato MRV, Magalhães Furtado F, Bertolucci CM, Severino AR, Souto EX, Ferreira Dos Santos TC, Dametto APF, Beltrame MP, Bacal NS, Avelar DMV, Fernandes RA, Gomes BE, da Costa ES, Santos BEFDS, Mendonça de Pontes R, Périco MDH, Gevert F, de Siqueira PFR, Marquezotti F, Wagner AOM, Soares ACCV, Duarte FB, Flowers ME, Vigorito AC.   +23 more
europepmc   +1 more source

Start, Stop, Rewind, Repeat—Cyclic Exposure of Adipose Stromal Cells‐derived Cartilage Organoids to Chondrogenic and Proliferative Cues to Achieve Scaled‐up and Customizable Bone Formation by Endochondral Ossification

Advanced Healthcare Materials, EarlyView.
This study exploits the plasticity of ASCs‐derived cartilage organoids which generate a perichondrial layer of MSCs when exposed to cyclic chondrogenic/proliferative cues. Using these organoids as building blocks, we develop (i) Phalange Shaped Tissue Engineered Cartilage (Pa‐TECs), recapitulating endochondral ossification suitable for the treatment of
Pablo Pfister, Emilien Lhospice, Andrés García‐García, Robert Paillaud, Sebastian Jung, Romain Schaller, Elisabeth A. Kappos, Claude Jaquiéry, Tarek Ismail, Dirk J. Schaefer, Michael de Wild, Ivan Martin, Alexandre Kaempfen, Arnaud Scherberich, Adrien Moya   +14 more
wiley   +1 more source

Identifying vascular stiffening-sensitive macrophages through integration of single-cell transcriptomics and imaging flow cytometry. [PDF]

Biophys Rep
Wang J, Wang J, Zhang Y, Xiong C, Liu SA, Kong Y, Zhou J, Wang X.   +7 more
europepmc   +1 more source

Engineered ETS1‐Nanoconjugate Restores Immune Homeostasis through Dual Immune‐Vascular Modulation in Relapsing and Progressive Multiple Sclerosis

Advanced Healthcare Materials, EarlyView.
The biomimetic nanoplatform IMNP (ETS1 pDNA/PBAE@ITP‐MM) undergoes targeted disassembly at inflammatory vascular sites to release the ETS1 plasmid (pETS1). This release initiates a cascade of effects that inhibit pathogenic pathways and support immune homeostasis. (Abbreviations: EndMT, endothelial‐to‐mesenchymal transition; EC, endothelial cell; TC, T
Feng Zhang, Si‐Bo Yang, Yan Luo, Xiao‐Di Sun, Bo‐Hao Chang, Da‐Qiang Zhou, Jun‐Jie Jiang, Ya‐Nan Li, Jie‐Hong Wu, Hui‐Juan Jin, Hang Yang, Hang Hu, Yi‐Fan Zhou, Bo Hu   +13 more
wiley   +1 more source

FlowLITE: A protocol to characterize and quantify total antibody isotypes in human plasma using flow cytometry. [PDF]

STAR Protoc
Hahm HJ, Nazzari AF, Babcock BR, Ghosn EEB.   +3 more
europepmc   +1 more source

Ferritin‐Doped Nanoparticles Triggered Tumor‐Specific Darkening for Enhanced Photothermal Tumor Ablation and Immune Activation

Advanced Healthcare Materials, EarlyView.
Ferritin‐doped hybrid nanoparticles triggered accurate tumor‐specific hemorrhage for enhanced in situ photothermal therapy and evoked anti‐tumor immunity. Notably, HFn/GA‐Fe specifically induced tumor hemorrhage 12 h after intravenous injection, resulting in visible color darkening. Through photoacoustic imaging, tumor vessels could be clearly observed
Haidong Zha, Xiao Liang, Long Xi, Jiamao Chen, Wenzhao Zhong, Zhen Yuan, Meng Xu, Ying Zheng   +7 more
wiley   +1 more source

Development of a novel flow cytometry multiplex immunoassay for the diagnosis of celiac disease simultaneously with autoimmune diabetes. [PDF]

Eur J Med Res
Bombicino SS, Trabucchi A, Sabljic AV, Marfía JI, Iacono RF, Valdez SN.   +5 more
europepmc   +1 more source

Electro‐Stimulated Graphene‐Polymer Nanocomposites Enable Wearable Patches With Feedback‐Controlled Drug Release

Advanced Healthcare Materials, EarlyView.
xx xx. ABSTRACT Stimuli‐responsive nanomaterials capable of spatiotemporal control over drug release are of nanocomposite patch (“e‐Medi‐Patch”) engineered from biodegradable polycaprolactone (PCL), graphene nanoplatelets, and a redox‐active therapeutic, niclosamide. The hierarchical composite integrates π‐π interactions between aromatic drug molecules
Santosh K. Misra, Ketan Dighe, Pranay Saha, Teresa Aditya, Muhammad S. Khan, Maha Alafeef, Parikshit Moitra, Dipanjan Pan   +7 more
wiley   +1 more source

Measuring Dedicator of Cytokinesis 8 (DOCK8) Expression as a Flow Cytometry Biomarker for DOCK8 Deficiency Detection

Maryam Moradi, Mahsa Yousefpour Marzbali, Nazanin Nazari, Zahra Chavoshzadeh, Seyed Alireza Mahdaviani, Mahnaz Sadeghi‐Shabestari, Reza Yazdani   +6 more
openalex   +1 more source