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Flow Cytometry of Apoptosis

Current Protocols in Cell Biology, 2003
AbstractCommon methods applicable to flow cytometry make it possible to: (1) identify and quantify dead or dying cells, (2) reveal a mode of cell death (apoptosis or necrosis), and (3) study mechanisms involved in cell death. Gross changes in cell morphology and chromatin condensation, which occur during apoptosis, can be detected by analysis with ...
Piotr, Pozarowski   +2 more
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Flow cytometry in melanoma

1988
Patients presenting with Stage I cutaneous melanoma have a highly variable clinical course. Because of this variability, numerous histologic and clinical prognostic factors have been developed, the most important of these factors being level of invasion, tumor thickness, ulceration, vertical growth phase, cell type, mitotic index, host inflammatory ...
S D, Bines   +3 more
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Flow cytometry in biotechnology

Applied Microbiology and Biotechnology, 2001
Flow cytometry is a general method for rapidly analyzing large numbers of cells individually using light-scattering, fluorescence, and absorbence measurements. The power of this method lies both in the wide range of cellular parameters that can be determined and in the ability to obtain information on how these parameters are distributed in the cell ...
M, Rieseberg   +3 more
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Basics of Flow Cytometry

2003
In summary, a beginner requires fundamental knowledge about flow cytometric instrumentation in order to effectively use this technology. It is important to remember that flow cytometers are very complex instruments that are composed of four closely related systems.
G, Radcliff, M J, Jaroszeski
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Flow Cytometry of Urine

Clinics in Laboratory Medicine, 1988
Flow cytometry is becoming an increasingly important tool for the evaluation of urine cytology. Although it has established a strong foothold in oncologic DNA analysis, studies of whole cells and cell markers are only in their infancy. In addition, potential diagnostic applications such as those involving binding of E.
A L, Landay, R A, Bray
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Flow Cytometry of Yeasts

Current Protocols in Cytometry, 1999
AbstractThis unit promotes increased interest in the use of flow cytometry in several new environments. The author provides protocols and descriptive detail on measurements of cell cycle, viability, respiratory activity, and β‐galactosidase activity. Many of these assays are described for other biological systems in CPC, but are now provided in detail ...
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Overview of Flow Cytometry and Fluorescent Probes for Flow Cytometry

2009
This chapter provides an introduction to the use of fluorescent probes in flow cytometry. Sample preparation for the use of surface labeling with antibodies as well as for the use of nucleic acid probes is discussed. The utility of cell sorting is also discussed.
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Automation in Flow Cytometry

Clinics in Laboratory Medicine
Automation in clinical flow cytometry has the potential to revolutionize the field by improving processes and enhancing efficiency and accuracy. Integrating advanced robotics and artificial intelligence, these technologies can streamline sample preparation, data acquisition, and analysis.
Giovanni, Insuasti-Beltran   +1 more
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Principles of flow cytometry

Cytometry, 1988
Flow cytometers can analyze a populations of cells, one at a time, at rates of 1,000 to 10,000 cells per second. They can provide distributions of parameters, not just the MCV. A broad variety of measurement parameters is available, ranging from simple sizing to esoteric measures of membrane fluidity or epitope density. The resulting analysis gives low
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Static and Flow Cytometry

2001
It has been known for over 50 years that the amount of nuclear chromatin (DNA) in malignant neoplasms differs from that of homologous normal cells (1). More recently, it has been shown that nuclear DNA content correlates with the clinical outcome of various human neoplasms including urologic malignancies (2-10).
R, Madeb, D, Pode, O, Nativ
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