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Methods in Cell Science, 2002
Flow cytometry is a technique for making quantitative measurements on single cells or cellular constituents at very high rates of speed. The value of flow cytometry has been likened to finding a needle in a haystack. A measurement of the average property of a haystack would fail to identify the needle. One alternative is to measure each particle in the
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Flow cytometry is a technique for making quantitative measurements on single cells or cellular constituents at very high rates of speed. The value of flow cytometry has been likened to finding a needle in a haystack. A measurement of the average property of a haystack would fail to identify the needle. One alternative is to measure each particle in the
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Flow Cytometry for the Immunotoxicologist
2018Assessing the immunotoxicity of xenobiotics by current regulatory testing has revealed compounds that can cause immunosuppression and stimulation. Flow cytometry is a cutting edge technique that can provide data on how toxicants can alter the quality and quantity of the immune response after exposure.
Scott T, Espenschied +2 more
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Transfusion Science, 1995
Clinical flow cytometry is a relatively new and rapidly growing medical technology. According to estimates, there were less than 1000 instruments in operation globally prior to 1985. Most of these instruments were used exclusively for research, and required dedicated facilities and operators with extensive backgrounds in electronics.
F F, Mandy, M, Bergeron, T, Minkus
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Clinical flow cytometry is a relatively new and rapidly growing medical technology. According to estimates, there were less than 1000 instruments in operation globally prior to 1985. Most of these instruments were used exclusively for research, and required dedicated facilities and operators with extensive backgrounds in electronics.
F F, Mandy, M, Bergeron, T, Minkus
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Biology of the Cell, 1986
Improvement in our knowledge in cellular biology is largely related to the use of new tools in quantitative cytology. Among them, flow cytometry was developed with numerous applications in the field of immunology including fundamental and applied research.
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Improvement in our knowledge in cellular biology is largely related to the use of new tools in quantitative cytology. Among them, flow cytometry was developed with numerous applications in the field of immunology including fundamental and applied research.
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2011
Laser technology has advanced tremendously since the first gas lasers were incorporated into early flow cytometers. Gas lasers have been largely replaced by solid-state laser technology, making virtually any desirable visible light wavelength available for flow cytometry. Multiwavelength, white light, and wavelength tunable lasers are poised to enhance
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Laser technology has advanced tremendously since the first gas lasers were incorporated into early flow cytometers. Gas lasers have been largely replaced by solid-state laser technology, making virtually any desirable visible light wavelength available for flow cytometry. Multiwavelength, white light, and wavelength tunable lasers are poised to enhance
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Instrumentation for flow cytometry
Journal of Immunological Methods, 2000Advances in fluidics, optics, electronics, lasers, computers and software have made flow cytometers considerably more complex, but also easier to use. Production of new fluorescent dyes has seen the development of more flexible instruments so that eight or more parameters of correlated data can be collected at once.
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2004
Cytokine flow cytometry (CFC) is a general term that applies to flow cytometric analysis of cells using anticytokine antibodies as markers of activation. The most common version of this technique is the intracellular staining of cytokines in cells that have been fixed and permeabilized after short-term in vitro activation.
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Cytokine flow cytometry (CFC) is a general term that applies to flow cytometric analysis of cells using anticytokine antibodies as markers of activation. The most common version of this technique is the intracellular staining of cytokines in cells that have been fixed and permeabilized after short-term in vitro activation.
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Current Protocols in Cytometry, 2004
AbstractLasers are the principle light sources of flow cytometers. Most modern benchtop instruments feature air‐cooled argon‐ion laser emitting 10‐25 mW of light at 488 nm. This unit covers the various types of lasers available and the qualities that make them suitable or unsuitable for use in flow cytometers.
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AbstractLasers are the principle light sources of flow cytometers. Most modern benchtop instruments feature air‐cooled argon‐ion laser emitting 10‐25 mW of light at 488 nm. This unit covers the various types of lasers available and the qualities that make them suitable or unsuitable for use in flow cytometers.
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Nature Methods, 2011
A new flow cytometry technology based on mass detection allows a very large number of parameters to be simultaneously measured in single cells.
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A new flow cytometry technology based on mass detection allows a very large number of parameters to be simultaneously measured in single cells.
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