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Flow cytometry, an overview

Methods in Cell Science, 2002
Flow cytometry is a technique for making quantitative measurements on single cells or cellular constituents at very high rates of speed. The value of flow cytometry has been likened to finding a needle in a haystack. A measurement of the average property of a haystack would fail to identify the needle. One alternative is to measure each particle in the
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Flow Cytometry

2011
Flow cytometry (FC) is defined as a method for the qualitative and quantitative measurement of biological and physical properties of cells and other particles suspended within a high-velocity fluid stream and passing through a laser beam in a single file.
B.-F. Alfonso, M. Al-Rubeai
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Flow cytometry in melanoma

1988
Patients presenting with Stage I cutaneous melanoma have a highly variable clinical course. Because of this variability, numerous histologic and clinical prognostic factors have been developed, the most important of these factors being level of invasion, tumor thickness, ulceration, vertical growth phase, cell type, mitotic index, host inflammatory ...
Steven D. Bines   +3 more
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Flow Cytometry: An Introduction

2004
A flow cytometer is an instrument that illuminates cells (or other particles) as they flow individually in front of a light source and then detects and correlates the signals from those cells that result from the illumination. In this chapter, each of the aspects of that definition will be described: the characteristics of cells suitable for flow ...
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Flow cytometry

BJOG: An International Journal of Obstetrics & Gynaecology, 1992
Van Dam, Peter André, Lowe, D.
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Basics of Flow Cytometry

2003
In summary, a beginner requires fundamental knowledge about flow cytometric instrumentation in order to effectively use this technology. It is important to remember that flow cytometers are very complex instruments that are composed of four closely related systems.
Gilbert Radcliff, Mark J. Jaroszeski
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Static and Flow Cytometry

2001
It has been known for over 50 years that the amount of nuclear chromatin (DNA) in malignant neoplasms differs from that of homologous normal cells (1). More recently, it has been shown that nuclear DNA content correlates with the clinical outcome of various human neoplasms including urologic malignancies (2-10).
Ofer Nativ, Dov Pode, Ralph Madeb
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Principles of flow cytometry

Cytometry, 1988
Flow cytometers can analyze a populations of cells, one at a time, at rates of 1,000 to 10,000 cells per second. They can provide distributions of parameters, not just the MCV. A broad variety of measurement parameters is available, ranging from simple sizing to esoteric measures of membrane fluidity or epitope density. The resulting analysis gives low
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Flow Cytometry of Yeasts

Current Protocols in Cytometry, 1999
AbstractThis unit promotes increased interest in the use of flow cytometry in several new environments. The author provides protocols and descriptive detail on measurements of cell cycle, viability, respiratory activity, and β‐galactosidase activity. Many of these assays are described for other biological systems in CPC, but are now provided in detail ...
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Flow Cytometry for the Immunotoxicologist

2018
Assessing the immunotoxicity of xenobiotics by current regulatory testing has revealed compounds that can cause immunosuppression and stimulation. Flow cytometry is a cutting edge technique that can provide data on how toxicants can alter the quality and quantity of the immune response after exposure.
Kymberly M. Gowdy   +2 more
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