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Field‐flow fractionation for nanoparticle characterization

Journal of Separation Science, 2021
AbstractThis review presents field‐flow fractionation: The elements of theory enable the link between the retention and the characteristics of the nanometer‐sized analytes to be highlighted. In particular, the nature of force and its way of being applied are discussed.
Gaëtane Lespes   +1 more
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Field-Flow Fractionation

Separation Science and Technology, 1976
Abstract Field-flow fractionation (FFF) is a separation method first described in 1966 (I). FFF is an elution technique, like chromatography, and the experimental sequence of pump, column, detector, and fraction collector is much like that used in chromatographic operations (2-4).
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Field-flow fractionation of macromolecules

Journal of Chromatography A, 1989
Field-flow fractionation (FFF) is a versatile family of techniques, applicable to macromolecules, colloids, and cell-sized particles. This paper focuses specifically on the applicability of FFF to macromolecules. Following a brief description of the principles of FFF, the characteristics of FFF that bear on its efficacy in separating macromolecules are
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Micro-Thermal Field-Flow Fractionation

Collection of Czechoslovak Chemical Communications, 2002
The effect of miniaturization of the separation channel on the performance of thermal field-flow fractionation (TFFF) is substantiated theoretically. The experiments carried out under carefully chosen experimental conditions proved the high performance of the separation of polymers within an extended range of molar masses from relatively low up to ...
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Sedimentation Field Flow Fractionation of Liposomes

Science, 1982
Quantitative analyses of the particle size distributions of liposomes were performed in 30 to 60 minutes by exponential-field sedimentation field flow fractionation. This gentle new separation method exhibits great potential for the high-resolution fractionation and the size or molecular weight analysis of a wide variety of biological macromolecules ...
J J, Kirkland, W W, Yau, F C, Szoka
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Development of Electrical Field-Flow Fractionation

Analytical Chemistry, 2000
Electrical field-flow fractionation (ElFFF) results for a series of polystyrene latex beads are presented. To first approximation, retention behavior can be related to conventional FFF theory, modified to account for a particle-wall repulsion effect.
, Tri, , Caldwell, , Beckett
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Sedimentation Field Flow Fractionation of DNA's

Science, 1984
Sedimentation field flow fractionation (SFFF) is a method for purifying and providing mass or size distribution information on samples containing particulates or soluble macromolecules. Since SFFF separations are based on simple physical phenomena related to first principles, molecular weight (or particle sizes) can be determined without ...
L E, Schallinger   +2 more
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Electrical Field-Flow Fractionation of Proteins

Science, 1972
Protein separation has been achieved by electrical field-flow fractionation, a heretofore unrealized separation technique. Some advantages of this method relative to electrophoresis are the low voltage required, the lack of adverse heating and support effects, and the existence of the method as an elution technique.
K D, Caldwell   +3 more
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Sedimentation Field Flow Fractionation: Applications

Science, 1982
Sedimentation field flow fractionation is a powerful, new, high-resolution separation method for a wide variety of colloids, micelles, particulates, and soluble macromolecules of biological interest. Advances in instrumentation allow sedimentation field flow fractionation operation with rotor speeds up to 32,000 revolutions per minute (∼85,
J J, Kirkland, W W, Yau
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Field flow fractionation in biomedical analysis

Biomedical Chromatography, 1991
AbstractSamples of biomedical interest which have been analysed by field‐flow fractionation techniques are surveyed. The list begins with whole cells and microorganisms, going through viruses, nucleic acids, cell fragments and organelles, down to proteins and their aggregates.
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