Results 111 to 120 of about 1,337,593 (318)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Effects of aggregation on the blood flow velocity field measured by a μPIV based technique [PDF]

open access: yes, 2009
This paper was presented at the 2nd Micro and Nano Flows Conference (MNF2009), which was held at Brunel University, West London, UK. The conference was organised by Brunel University and supported by the Institution of Mechanical Engineers, IPEM, the ...
2nd Micro and Nano Flows Conference (MNF2009)   +3 more
core  

Dynamics of semiflexible polymers in a flow field

open access: yesPhysical Review E, 2006
We present a novel method to investigate the dynamics of a single semiflexible polymer, subject to anisotropic friction in a viscous fluid. In contrast to previous approaches, we do not rely on a discrete bead-rod model, but introduce a suitable normal mode decomposition of a continuous space curve.
Munk, Tobias   +3 more
openaire   +7 more sources

Why human connection is the true metric of research success

open access: yesFEBS Open Bio, EarlyView.
Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan   +3 more
wiley   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

open access: yesFEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho   +4 more
wiley   +1 more source

CFD Investigation on 3-Dimensional Interference of a Five-Hole Probe in an Automotive Wheel Arch [PDF]

open access: yes, 2010
Detailed flowmeasurements are essential for analysing flow structures found in confined spaces, particularly in various automotive applications. These measurements will be extremely helpful in solving flow dependent complexities.
Palmer, E.   +8 more
core   +1 more source

Effect of Parameters of Ditch Geometry on the Uniformity of Water Filling in Ship Lock Chambers

open access: yesJournal of Marine Science and Engineering
The design of ditch structures in ship locks has an important influence on reducing water flow energy, improving the uniformity of water filling in lock chambers, and reducing the force acting on mooring ships.
Qiaoyi Hu, Yun Li, Long Zhu
doaj   +1 more source

Field-flow fractionation [PDF]

open access: yesAnalytical and Bioanalytical Chemistry, 2014
S Kim R, Williams, Karin D, Caldwell
openaire   +2 more sources

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

open access: yesFEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer   +7 more
wiley   +1 more source

Hydrodynamic Modeling and Mathematical Simulation on Flow Field and Inclusion Removal in a Seven-Strand Continuous Casting Tundish with Channel Type Induction Heating

open access: yesMetals, 2018
The tundish with heating instrumentation is attracting more and more attention in continuous casting processes for maintaining a pre-determined constant pouring temperature under a given casting speed, which is beneficial for an improved and consistent ...
Haiyan Tang   +5 more
doaj   +1 more source

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