Results 81 to 90 of about 315,978 (276)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Integer Lattice Gas with Monte Carlo collision operator recovers the entropic lattice Boltzmann method with Poisson distributed fluctuations

, 2017
We are examining a new kind of lattice gas that closely resembles modern lattice Boltzmann methods. This new kind of lattice gas, that we call a Monte Carlo Lattice Gas, has interesting properties that shed light on the origin of the multi-relaxation ...
Blommel, Thomas, Wagner, Alexander J.
core   +1 more source

Adaptive phase estimation is more accurate than non-adaptive phase estimation for continuous beams of light [PDF]

, 2004
We consider the task of estimating the randomly fluctuating phase of a continuous-wave beam of light. Using the theory of quantum parameter estimation, we show that this can be done more accurately when feedback is used (adaptive phase estimation) than ...
A. Benssousan, A. S. Holevo, A. Yariv, C. W. Gardiner, D. T. Pope, D. W. Berry, H. M. Wiseman, H. P. Yuen, H. P. Yuen, J. H. Shapiro, J. H. Shapiro, J. H. Shapiro, L. Mandel, N. K. Langford, S.M. Barnett, T. P. McGarty   +15 more
core   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Rate of photosynthetic induction in fluctuating light varies widely among genotypes of wheat. [PDF]

, 2019
Crop photosynthesis and yield are limited by slow photosynthetic induction in sunflecks. We quantified variation in induction kinetics across diverse genotypes of wheat for the first time.
Buckley, Thomas N, Merchant, Andrew M, Richards, Richard A, Salter, William T, Trethowan, Richard   +4 more
core  

Multifractal analysis of light scattering-intensity fluctuations

Physical Review E, 2009
We provide a simple interpretation of non-Gaussian nature of the light scattering-intensity fluctuations from an aging colloidal suspension of Laponite using the multiplicative cascade model, Markovian method, and volatility correlations. The cascade model and Markovian method enable us to reproduce most of recent empirical findings: long-range ...
Shayeganfar, F., Jabbari-Farouji, S., Movahed, M.S., Jafari, G.R., Tabar, M.R.R.   +4 more
openaire   +2 more sources

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Elastic energy loss and longitudinal straggling of a hard jet [PDF]

, 2008
The elastic energy loss encountered by jets produced in deep-inelastic scattering (DIS) off a large nucleus is studied in the collinear limit. In close analogy to the case of (non-radiative) transverse momentum broadening, which is dependent on the ...
A. Majumder, B. G. Zakharov, B. G. Zakharov, J. D. Jackson, J. I. Kapusta   +4 more
core   +2 more sources

Real‐World Performance of CSF Kappa Free Light Chains in the 2024 McDonald Criteria

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Kappa free light chains (KFLCs) in the cerebrospinal fluid (CSF) have a similar performance to CSF‐restricted oligoclonal bands (OCB) for multiple sclerosis (MS) diagnosis. To help with implementation, we set out to resolve several remaining uncertainties: (1) performance in a real‐world cohort and the 2024 McDonald criteria; (2 ...
Maya M. Leibowitz, Ryan Cooper, Stefania Kaninia, Kathryn I. Challis, Will Greenway, Maria Bonello, Mahmoud Elbahnasawi, Deborah T. Shode, Dominika Gajdasik, Victor Iliev, Alison M. E. Whitelegg, Ian Galea   +11 more
wiley   +1 more source

Remote Assessment of Ataxia Severity in SCA3 Across Multiple Centers and Time Points

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Spinocerebellar ataxia type 3 (SCA3) is a genetically defined ataxia. The Scale for Assessment and Rating of Ataxia (SARA) is a clinician‐reported outcome that measures ataxia severity at a single time point. In its standard application, SARA fails to capture short‐term fluctuations, limiting its sensitivity in trials.
Marcus Grobe‐Einsler, Vivian Maas, Arian Taheri Amin, Jennifer Faber, Tamara Schaprian, Katharina Hill, Matthis Synofzik, Lisa H. Graf, Heike Jacobi, Manuela Lima, Ana F. Ferreira, Bart P. van de Warrenburg, Ilse H. J. Willemse, Dagmar Timmann, Andreas Thieme, Paola Giunti, Hector Garcia‐Moreno, Magda M. Santana, Jeannette Hübener‐Schmid, Elin H. Davies, Thomas Klockgether   +20 more
wiley   +1 more source