Results 71 to 80 of about 1,062,005 (252)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Three-Dimensional Fluid–Structure Interaction Case Study on Elastic Beam

Journal of Marine Science and Engineering, 2020
A three-dimensional T-shaped flexible beam deformation was investigated using model experiments and numerical simulations. In the experiment, a beam was placed in a recirculating water channel with a steady uniform flow in the inlet.
Mahdi Tabatabaei Malazi, Emir Taha Eren, Jing Luo, Shuo Mi, Galip Temir   +4 more
doaj   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Fluid-Structure Interaction Analysis of Parachute Finite Mass Inflation

International Journal of Aerospace Engineering, 2016
Parachute inflation is coupled with sophisticated fluid-structure interaction (FSI) and flight mechanic behaviors in a finite mass situation. During opening, the canopy often experiences the largest deformation and loading.
Xinglong Gao, Qingbin Zhang, Qiangang Tang   +2 more
doaj   +1 more source

Relativistic theories of interacting fields and fluids

, 2001
We investigate divergence-type theories (DTT) describing the dissipative interaction between a field and a fluid. We look for theories which, under equilibrium conditions, reduce to the theory of a Klein-Gordon scalar field and a perfect fluid.
A. Albrecht, A. D. Dolgov, A. D. Dolgov, A. D. Linde, A. H. Guth, B. L. Hu, C. W. Misner, D. Boyanovsky, D. Jou, E. Calzetta, E. Calzetta, E. Calzetta, E. Calzetta, E. Copeland, E. W. Kolb, Esteban Calzetta, F. Finelli, H. Kreiss, H. Kreiss, I. Liu, J. Lidsey, J. Stewart, L. F. Abbott, L. Kofman, L. Kofman, L. Landau, L. P. Chimento, L. P. Chimento, L. P. Kadanoff, M. E. Carrington, M. Turner, M. Turner, Marc Thibeault, R. Courant, R. Geroch, R. H. Brandenburger, S. A. Ramsey, S. A. Ramsey, S. Jeon, S. Jeon, S. Jeon, S. Khlebnikov, S. Khlebnikov, V. Mukhanov, W. A. Hiscock, W. A. Hiscock, W. Hiscock, W. Israel, W. Israel, W. Zimdahl, W. Zimdahl, W. Zimdahl, W. Zimdahl, Y. Hu   +53 more
core   +1 more source

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Fluid-particle-structure interaction in single shot peening

Scientific Reports
Shot peening is a widely used cold-working process. Physical phenomena of shot peening are analyzed using the developed fluid-particle-structure coupled solver. The influences of the flow field and shot peening parameters such as the shot impact velocity
Yusuke Mizuno, Takashi Misaka, Yoshiyuki Furukawa   +2 more
doaj   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Fast Solvers for Unsteady Thermal Fluid Structure Interaction

, 2014
We consider time dependent thermal fluid structure interaction. The respective models are the compressible Navier-Stokes equations and the nonlinear heat equation. A partitioned coupling approach via a Dirichlet-Neumann method and a fixed point iteration
Birken, Philipp, Gleim, Tobias, Kuhl, Detlef, Meister, Andreas   +3 more
core