Results 31 to 40 of about 44,669 (268)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Analysis of Aerodynamic Noise Characteristics of High-Speed Train Pantograph with Different Installation Bases

Applied Sciences, 2019
The high-speed-train pantograph is a complex structure that consists of different rod-shaped and rectangular surfaces. Flow phenomena around the pantograph are complicated and can cause a large proportion of aerodynamic noise, which is one of the main ...
Yongfang Yao, Zhenxu Sun, Guowei Yang, Wen Liu, Prasert Prapamonthon   +4 more
doaj   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Research on aerodynamic optimization of high-speed train’s slipstream

Engineering Applications of Computational Fluid Mechanics, 2020
Slipstream severely affects the safety of trackside workers and equipment. With use of profile superimposition method and vehicle modeling method, parametrization of the whole train is carried out. Then, a slipstream optimization study has been performed,
Zhenxu Sun, Shuanbao Yao, Guowei Yang
doaj   +1 more source

CSF Monoamine Metabolites and Cognitive Trajectory in Early Parkinson's Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Imaging and postmortem studies indicate that abnormalities in monoaminergic neurotransmission contribute to cognitive impairment in Parkinson's disease (PD). However, it remains uncertain if cerebrospinal fluid (CSF) monoamine metabolites can serve as biomarkers of cognitive decline in early PD.
Jing‐Yu Shao, Rong Li, Yu‐Xiang Jiao, Kai Ma, Hong‐Qi Yang, Zheng‐Yu Sun, Jie‐Wen Zhang, Shuai Chen   +7 more
wiley   +1 more source

Analysis of the Transonic Buffet Characteristics of Stationary and Pitching OAT15A Airfoil

Applied Sciences
Transonic buffet flow is a classical complex and unstable flow that has a negative effect on aircraft fly safety. Therefore, it is crucial to study the unsteady characteristics of buffet flow. The numerical analysis method is very useful in achieving the
Xueyuan Nie, Guannan Zheng, Lianyi Wei, Chengde Huang, Guowei Yang, Zhanling Ji   +5 more
doaj   +1 more source

Early Clinical, Imaging, and Pathological Characteristics of SRPK3/TTN‐Digenic Myopathy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective SRPK3/TTN‐digenic myopathy was recently established as a skeletal muscle myopathy caused by digenic inheritance. This study characterizes the early clinical presentation of SRPK3/TTN‐digenic myopathy in one previously reported and seven newly identified pediatric patients.
Rotem Orbach, Sandra Donkervoort, Carola Hedberg‐Oldfors, Giovanni Baranello, Dimah Saade, Precilla D'Souza, Ruchee Patel, Eva Michael, A. Reghan Foley, Diana Bharucha‐Goebel, S. Jin Haugland, Meghan McAnally, Omer Abdul Hamid, Katherine Chao, Ellen F. Macnamara, Alan H. Beggs, Anna Sarkozy, Juliane Mueller, Steven A. Moore, Richard S. Finkel, Cynthia J. Tifft, Francesco Muntoni, Anders Oldfors, Carsten G. Bönnemann   +23 more
wiley   +1 more source

Data-Driven Adaptive Control of Transonic Buffet via Localized Morphing Skin

Aerospace
Transonic shock buffet, characterized by large-amplitude self-sustained shock oscillations arising from shock wave/boundary layer interactions, poses significant challenges to aircraft handling quality and structural integrity.
Yuchen Zhang, Lianyi Wei, Yiqiu Jin, Han Tang, Guannan Zheng, Guowei Yang   +5 more
doaj   +1 more source

Fluid-solid Coupling Analysis of the Pressure Expansion of Packers

Journal of Physics: Conference Series, 2020
Abstract For the packers, the pressure expansion process and differential pressure loading process have been simulated by the fluid-solid coupling finite element method. The results show that under the internal pressure load of 10MPa at both ends of the hydraulic oil, the structure of the packer can be fully expanded, and the simulated ...
Lanwen Wang, Junxu Ge, Xuanyu Sheng
openaire   +1 more source

Planar Solid‐State Nanopores Toward Scalable Nanofluidic Integration Based on CMOS Technology

Advanced Engineering Materials, EarlyView.
We present a scalable silicon‐based fabrication strategy for planar solid‐state nanopores to enable their integration with complex nanofluidic systems. Prototype devices demonstrate normal voltage‐current characteristics, good noise performance, and appreciable streaming currents. Our CMOS‐compatible fabrication process offers precise geometric control
Ngan Hoang Pham, Chenyu Wen, Mohammad Hadi Khaksaran, Won‐Yong Lee, Javier Cruz, Klas Hjort, Dongping Wu, Shi‐Li Zhang   +7 more
wiley   +1 more source