Results 161 to 170 of about 1,767,668 (275)

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Generating evidence using electronic alerts during routine care: a fully automated randomized controlled trial of oral fluid restriction in acute heart failure (THIRST alert trial). [PDF]

Eur Heart J Digit Health
Chen Y, Higgins D, Jani Y, Saleem N, Chafer K, McFarlane E, Roberts T, Harris S, Williams B, Sydes MR, Asselbergs FW, Shah AD, Lumbers RT.   +12 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Postoperative fluid restriction to prevent delayed hyponatremia after endoscopic transsphenoidal surgery. [PDF]

Neuro Oncol
Klaassen D, Mok S, Hwang JY, Blount SL, Williams KJ, Fong BM, Chicoine MR, Dacey RG, Farrell NF, Osbun JW, Rich KM, Roland LT, Schneider JS, Zipfel GJ, Luo C, Kim AH, Silverstein JM.   +16 more
europepmc   +1 more source

COMPLICATIONS OF FLUID RESTRICTION IN THE PERINATAL PERIOD [PDF]

Pediatric Research, 1998
V Kavvadia, A Greenough, G Dimitriou, M Forsling   +3 more
openaire   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Urine Electrolyte to Predict Treatment Failure of Fluid Restriction in Syndrome of Inappropriate Antidiuresis: A Prospective Multicenter Study. [PDF]

Kidney360
Chienwichai K, Jiwakanon S, Chaiviriyawong K, Wattanakul J, Rojnsaengroung W, Manasuth S, Sangkaew S, Chang A, Mongkolrattanakul P.   +8 more
europepmc   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

24 h severe fluid restriction increases a biomarker of renal injury in healthy males. [PDF]

Eur J Appl Physiol
Juett LA, Funnell MP, Carroll HA, James LJ, Mears SA.   +4 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source