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Leaf diseases, such as powdery mildew and leaf rust, frequently infect barley plants and severely affect the economic value of malting barley. Early detection of barley diseases would facilitate the timely application of fungicides. In a field experiment,
Kang Yu+5 more
doaj +1 more source
Light-dependent changes in outer segment free-Ca2+ concentration in salamander cone photoreceptors. [PDF]
Simultaneous measurements of photocurrent and outer segment Ca2+ were made from isolated salamander cone photoreceptors. While recording the photocurrent from the inner segment, which was drawn into a suction pipette, a laser spot confocal technique was ...
Barnes+46 more
core +2 more sources
著者は第4報1)及び第7報2)に於て酸化還元滴定の螢光指示藥としてα-Naphthoflavone, Rhodamine B及びFluoresceinの使用に就て報告せり.其後更にPhosphineを使用して紫外線下にて鐡及び過酸化水素の過マンガン酸滴定,及び亞砒酸の臭素滴定を試み,又鹽素滴定にFluorescein及びRhodamine Bを螢光指示藥として試みたるにより其結果を報告す.
openaire +20 more sources
In this work, we reveal how different enzyme binding configurations influence the fluorescence decay of NAD(P)H in live cells using time‐resolved anisotropy imaging and fluorescence lifetime imaging microscopy (FLIM). Mathematical modelling shows that the redox states of the NAD and NADP pools govern these configurations, shaping their fluorescence ...
Thomas S. Blacker+8 more
wiley +1 more source
We report on the recent advancements in the sensing of proteins, both directly and with the use of a fluorescent probe, through the use of Fluorophore-Induced Plasmonic Current (FIPC). FIPC are a phenomenon where a fluorophore or excited state species is
Daniel R. Pierce, Chris D. Geddes
doaj +1 more source
Three-dimensional virtual refocusing of fluorescence microscopy images using deep learning
Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D fluorescence image ...
Ben-David, Eyal+7 more
core +1 more source
Neutrophil deficiency increases T cell numbers at the site of tissue injury in mice
In wild‐type mice, injury or acute inflammation induces neutrophil influx followed by macrophage accumulation. Mcl1ΔMyelo (neutrophil‐deficient) mice lack neutrophils, and in response to muscle injury show fewer macrophages and exhibit strikingly elevated T‐cell numbers, primarily non‐conventional “double‐negative” (DN) αβ and γδ T cells.
Hajnalka Halász+6 more
wiley +1 more source
Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells
We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera.
Barnea, Itay+3 more
core +1 more source
The yeast mitoribosome assembly factor Rsm22 contains a [4Fe‐4S] cluster that is matured by the mitochondrial iron–sulfur cluster assembly (ISC) machinery. Defects in ISC components result in impaired mitochondrial protein synthesis due to a mitoribosome assembly defect.
Ulrich Mühlenhoff+4 more
wiley +1 more source