Results 71 to 80 of about 1,945,444 (315)

Exploring conformational dynamics of flavoenzymes with flavin fluorescence relaxation spectroscopy [PDF]

, 2002
Research described in this thesis was aimed at gaining more insight into the active-site dynamics of dimeric flavoproteins by means of fluorescence relaxation spectroscopy.
Berg, P.A.W., van den, van den Berg, P.A.W.   +1 more
core  

Quantifying nuclear wide chromatin compaction by phasor analysis of histone Förster resonance energy transfer (FRET) in frequency domain fluorescence lifetime imaging microscopy (FLIM) data

Data in Brief, 2020
The nanometer spacing between nucleosomes throughout global chromatin organisation modulates local DNA template access, and through continuous dynamic rearrangements, regulates genome function [1].
Zhen Liang, Jieqiong Lou, Lorenzo Scipioni, Enrico Gratton, Elizabeth Hinde   +4 more
doaj   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source

Time-resolved fluorescence-based europium-derived probes for peroxidase bioassays, citrate cycle imaging and chirality sensing [PDF]

, 2005
The dissertation describes the development of europium-derived fluorescence probes for biological substances in aqueous solution. A scheme is shown in Fig. 7.1.
Lin, Zhihong
core   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Fluorescence Lifetime of PDT Photosensitizers [PDF]

, 2008
Photodynamic therapy (PDn is an effective treatment option for various types of invasive tumors, the efficacy of which depends strongly on selective cell uptake and selective excitation of the tumor, which relies on proper dosage.
Russell, Jennifer
core  

Translational and rotational motions of albumin sensed by a non-covalent associated porphyrin under physiological and acidic conditions: a fluorescence correlation spectroscopy and time resolved anisotropy study. [PDF]

, 2008
The interaction between a free-base, anionic water-soluble porphyrin, TSPP, and the drug carrier protein, bovine serum albumin (BSA) has been studied by time-resolved fluorescence anisotropy (TRFA) and fluorescence correlation spectroscopy (FCS) at two ...
Costa, S.M., Hoek, A., van, Borst, J.W., Visser, A.J.W.G., Andrade, S.M.   +4 more
core   +1 more source

Advancing the Frontiers of Biophysical Research and Cellular Dynamics: Single-Molecule Tracking for Live Cells—A Deep Dive

Biophysica
This article addresses a current point of contention in the field of single-molecule/single-particle tracking, as well as the relevant literature, and supplements it with some published cell-based experiments to illustrate our conclusions and known ...
Shih-Chu Jeff Liao, Beniamino Barbieri, Gerd Baumann, Zeno Földes-Papp   +3 more
doaj   +1 more source

Investigation of Leaf Diseases and Estimation of Chlorophyll Concentration in Seven Barley Varieties Using Fluorescence and Hyperspectral Indices

Remote Sensing, 2013
Leaf diseases, such as powdery mildew and leaf rust, frequently infect barley plants and severely affect the economic value of malting barley. Early detection of barley diseases would facilitate the timely application of fungicides. In a field experiment,
Kang Yu, Georg Leufen, Mauricio Hunsche, Georg Noga, Xinping Chen, Georg Bareth   +5 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source