This article addresses a current point of contention in the field of single-molecule/single-particle tracking, as well as the relevant literature, and supplements it with some published cell-based experiments to illustrate our conclusions and known ...
Shih-Chu Jeff Liao +3 more
doaj +1 more source
Laser-induced fluorescence determination of temperatures in low pressure flames [PDF]
Rensberger KJ, Jeffries JB, Copeland RA, Kohse-Höinghaus K, Wise ML, Crosley DR. Laser-induced fluorescence determination of temperatures in low pressure flames. Applied Optics.
Jeffries, Jay B. +5 more
core
A fluctuation method to quantify in vivo fluorescence data [PDF]
Quantitative in vivo measurements are essential for developing a predictive understanding of cellular behavior. Here we present a technique that converts observed fluorescence intensities into numbers of molecules.
Y Uri Alon +9 more
core +1 more source
Emerging experimental and computational methods for studying redox‐regulated structural transitions
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass +2 more
wiley +1 more source
Red-Shifted FRET Biosensors for High-Throughput Fluorescence Lifetime Screening
We have developed fluorescence resonance energy transfer (FRET) biosensors with red-shifted fluorescent proteins (FP), yielding improved characteristics for time-resolved (lifetime) fluorescence measurements.
Tory M. Schaaf +9 more
doaj +1 more source
Deriving sun-induced chlorophyll fluorescence from airborne based spectrometer data [PDF]
Sun-induced chlorophyll fluorescence (Fs) is a promising parameter for remote measuring plant photosynthesis. It has been demonstrated that Fs at cell and leaf level is strongly related to photosynthesis.
Schaepman, Michael E; https://orcid.org/ +10 more
core +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
In Vivo Single-Cell Detection of Metabolic Oscillations in Stem Cells
Through the use of bulk measurements in metabolic organs, the circadian clock was shown to play roles in organismal energy homeostasis. However, the relationship between metabolic and circadian oscillations has not been studied in vivo at a single-cell ...
Chiara Stringari +7 more
doaj +1 more source
BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing
Summary: The transcription factor BMAL1 is a core element of the circadian clock that contributes to cyclic control of genes transcribed by RNA polymerase II.
Marlene Cervantes +5 more
doaj +1 more source
Translophagy—A potential link between autophagy impairment and translational errors
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk +11 more
wiley +1 more source

