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Fundamentals of Fluorescence and Fluorescence Microscopy

2003
This chapter discusses the fundamental physics of fluorescence. The application of fluorescence to microscopy represents an important transition in the development of microscopy, particularly as it applies to biology. It enables quantitating the amounts of specific molecules within a cell, determining whether molecules are complexing on a molecular ...
openaire   +5 more sources

Enhancement and quenching of single-molecule fluorescence.

Physical Review Letters, 2006
We present an experimental and theoretical study of the fluorescence rate of a single molecule as a function of its distance to a laser-irradiated gold nanoparticle.
P. Anger, Palash Bharadwaj, L. Novotný
semanticscholar   +1 more source

Fluorescence and autofluorescence

Best Practice & Research Clinical Gastroenterology, 1999
Fluorescence detection is one of a series of new spectroscopic techniques currently developed for implementation in endoscopy. This technology is likely to significantly enhance our ability to detect minute lesions and to predict the histology of certain macroscopic lesions.
J. Haringsma, G.N.J. Tytgat
openaire   +3 more sources

Fluorescence Microscopy and Fluorescent Probes

1996
Invited Review Lectures: Recent Developments in Fluorescence Microscopy Long-Lived Metal-Ligand Probes Three-Photon Excitation and Optical Control of Excited State Population J.R. Lakowicz, et al. Computer Simulations of the Behavior of Hydrophobic Probe Molecules in Lipid Bilayers H.C. Gerritsen, Y.K. Levine.
Fluorescent Probes, Jan Slavík
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Fluorescence Microscopy and Fluorescence Probes

Microscopy and Microanalysis, 1998
Fluorescence Microscopy and Fluorescence Probes, edited by Jan Slavik, 1996. Plenum Press, New York and London. 306 pages. (hardback, $95)This volume is a compilation of abstracts of papers presented at the Fluorescence Microscopy and Fluorescence Probes Conference held in Prague in June 1995.
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Molecular Fluorescence in Citric Acid-Based Carbon Dots

, 2017
Nitrogen-doped carbon dots synthesized from citric acid as a carbon precursor have recently been considered to contain fluorescent derivatives of citrazinic acid, which contribute to their emission in the blue spectral range.
J. Schneider   +6 more
semanticscholar   +1 more source

Graphitic Nitrogen Triggers Red Fluorescence in Carbon Dots.

ACS Nano, 2017
Carbon dots (CDs) are a stable and highly biocompatible fluorescent material offering great application potential in cell labeling, optical imaging, LED diodes, and optoelectronic technologies.
Kateřina Holá   +6 more
semanticscholar   +1 more source

Fluorescence Microscopy

Current Protocols in Immunology, 1998
AbstractAbsorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular ...
openaire   +5 more sources

Fluorescent proteins for single‐molecule fluorescence applications

Journal of Biophotonics, 2008
AbstractWe present single‐molecule fluorescence data of fluorescent proteins GFP, YFP, DsRed, and mCherry, a new derivative of DsRed. Ensemble and single‐molecule fluorescence experiments proved mCherry as an ideally suited fluorophore for single‐molecule applications, demonstrated by high photostability and rare fluorescence‐intensity fluctuations ...
Seefeldt, Britta   +6 more
openaire   +3 more sources

Environmental effects on vibronic band intensities in pyrene monomer fluorescence and their application in studies of micellar systems

, 1977
The fluorescence intensities for various vibronic fine structures in the pyrene monomer fluorescence show strong solvent dependence. In the presence of polar solvents, there is a significant enhancement in the intensity of the 0--0 vibronic band at the ...
K. Kalyanasundaram, J. K. Thomas
semanticscholar   +1 more source

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