Generation of photonic entanglement in green fluorescent proteins [PDF]
Nature Communications, 2017 Quantum-enhanced applications such as quantum spectroscopy of biological samples could take advantage from in situ generation of quantum states of light.
Siyuan Shi, Prem Kumar, Kim Fook Lee
doaj +3 more sources
Split Green Fluorescent Proteins: Scope, Limitations, and Outlook. [PDF]
Annu Rev Biophys, 2019 Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to ...
Romei MG, Boxer SG.
europepmc +4 more sources
Imaging individual green fluorescent proteins [PDF]
Nature, 1997 Recent advances in fluorescence microscopy techniques have allowed the video-time imaging of single molecules of fluorescent dyes covalently bound to proteins in aqueous environments1. However, the techniques have not been exploited fully because proteins can be difficult to label, and dye modification may cause partial or complete loss of activity ...
D. Pierce, N. Hom-Booher, R. Vale
semanticscholar +5 more sources
Red fluorescent proteins engineered from green fluorescent proteins. [PDF]
Proc Natl Acad Sci U S A, 2023 Fluorescent proteins (FPs) form a fluorophore through autocatalysis from three consecutive amino acid residues within a polypeptide chain. The two major groups, green FPs (GFPs) and red FPs (RFPs), have distinct fluorophore structures; RFPs have an extended π-conjugation system with an additional double bond.
Imamura H +4 more
europepmc +3 more sources
Very bright green fluorescent proteins from the Pontellid copepod Pontella mimocerami. [PDF]
PLoS ONE, 2010 Fluorescent proteins (FP) homologous to the green fluorescent protein (GFP) from the jellyfish Aequorea victoria have revolutionized biomedical research due to their usefulness as genetically encoded fluorescent labels. Fluorescent proteins from copepods
Marguerite E Hunt +3 more
doaj +2 more sources
Incomplete proteasomal degradation of green fluorescent proteins in the context of tandem fluorescent protein timers. [PDF]
Mol Biol Cell, 2016 Tandem fluorescent protein timers (tFTs) report on protein age through time-dependent change in color, which can be exploited to study protein turnover and trafficking.
Khmelinskii A +8 more
europepmc +2 more sources
The coherent dynamics of photoexcited green fluorescent proteins [PDF]
Physical Review Letters, 2001 The coherent dynamics of vibronic wave packets in the green fluorescent protein is reported. At room temperature the non-stationary dynamics following impulsive photoexcitation displays an oscillating optical transmissivity pattern with components at 67 ...
Beltram, Fabio +8 more
core +5 more sources
CemOrange2 fusions facilitate multifluorophore subcellular imaging in C. elegans. [PDF]
PLoS ONE, 2019 Due to its ease of genetic manipulation and transparency, Caenorhabditis elegans (C. elegans) has become a preferred model system to study gene function by microscopy.
Brian J Thomas +14 more
doaj +4 more sources
Improved split fluorescent proteins for endogenous protein labeling [PDF]
Nature Communications, 2017 Split fluorescent proteins (FPs) have been widely used to visualise proteins in cells. Here the authors develop a screen for engineering new split FPs, and report a yellow-green split-mNeonGreen2 with reduced background, a red split-sfCherry2 for ...
Siyu Feng +5 more
doaj +3 more sources
Some Secrets of Fluorescent Proteins: Distinct Bleaching in Various Mounting Fluids and Photoactivation of cyan fluorescent proteins at YFP-Excitation [PDF]
, 2011 Background The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking specific proteins or cells
Johannes Schmid, Naila Malkani
core +23 more sources