Results 1 to 10 of about 3,922,099 (302)
Imaging individual green fluorescent proteins [PDF]
Nature, 1997 Recent advances in fluorescence microscopy techniques have allowed the video-time imaging of single molecules of fluorescent dyes covalently bound to proteins in aqueous environments1. However, the techniques have not been exploited fully because proteins can be difficult to label, and dye modification may cause partial or complete loss of activity ...
D W, Pierce, N, Hom-Booher, R D, Vale
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Photoacid Dynamics in the Green Fluorescent Protein
Annual Review of Physical Chemistry, 2023 The photoacid dynamics of fluorescent proteins include both electronic excited- and ground-state mechanisms of proton transfer. The associated characteristic timescales of these reactions range over many orders of magnitude, and the tunneling, barrier crossing, and relevant thermodynamics have in certain cases been linked to coherent nuclear motion ...
Jasper J, van Thor, Paul M, Champion
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Evaluation of chemical fluorescent dyes as a protein conjugation partner for live cell imaging.
PLoS ONE, 2014 To optimize live cell fluorescence imaging, the choice of fluorescent substrate is a critical factor. Although genetically encoded fluorescent proteins have been used widely, chemical fluorescent dyes are still useful when conjugated to proteins or ...
Yoko Hayashi-Takanaka +4 more
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Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
Microbial Cell Factories, 2023 Background Several fusion tags for separation handle have been developed, but the fused tag for simply and cheaply separating the target protein is still lacking.
Xiaomei He +6 more
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Development of small fluorescent probes for the analysis of autophagy kinetics
iScience, 2023 Summary: Autophagy is a dynamic process that degrades subcellular constituents, and its activity is measured by autophagic flux. The tandem proteins RFP-GFP-LC3 and GFP-LC3-RFP-LC3ΔG, which enable the visualization of autophagic vacuoles of different ...
Hajime Tajima Sakurai +9 more
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The molecular structure of green fluorescent protein [PDF]
Nature Biotechnology, 1996 The crystal structure of recombinant wild-type green fluorescent protein (GFP) has been solved to a resolution of 1.9 A by multiwavelength anomalous dispersion phasing methods. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder.
F, Yang, L G, Moss, G N, Phillips
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Three-Color Imaging Using Fluorescent Proteins in Living Zebrafish Embryos
BioTechniques, 2001 The zebrafish embryo is especially valuable for cell biological studies because of its optical clarity. In this system, use of an in vivo fluorescent reporter has been limited to green fluorescent protein (GFP).
Kenneth R. Finley +2 more
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BiophysicaFluorescent proteins find application as biocompatible, genetically encoded labels for visualization of living organisms tissues. Green fluorescent proteins (GFPs) are the most diverse, but proteins with red fluorescence have advantages, such as lower ...
Vladimir B. Krapivin +2 more
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Sub-cellular trafficking of phytochemicals explored using auto-fluorescent compounds in maize cells
BMC Plant Biology, 2003 Background Little is known regarding the trafficking mechanisms of small molecules within plant cells. It remains to be established whether phytochemicals are transported by pathways similar to those used by proteins, or whether the expansion of ...
Grotewold Erich +2 more
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Green Fluorescent Protein Glows Gold [PDF]
Cell, 2008 The awarding of this year's Nobel Prize in Chemistry to Osamu Shimomura, Martin Chalfie, and Roger Tsien for their discovery and development of green fluorescent protein earns this humble jellyfish protein a place of honor in the biology research hall of fame.
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