Results 11 to 20 of about 510,204 (262)
Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein [PDF]
Journal of the American Chemical Society, 2017 Green fluorescent protein, GFP, has revolutionized biology, due to its use in bioimaging. It is widely accepted that the protein environment makes its chromophore fluoresce, whereas the fluorescence is completely lost when the native chromophore is taken out of GFP.
Annette Svendsen +4 more
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eLife, 2022 Using a neural network to predict how green fluorescent proteins respond to genetic mutations illuminates properties that could help design new proteins.
Grzegorz Kudla, Marcin Plech
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Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]
The Journal of Physical Chemistry B, 1999 The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G. +3 more
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Imaging the Environment of Green Fluorescent Protein [PDF]
Biophysical Journal, 2002 An emerging theme in cell biology is that cell surface receptors need to be considered as part of supramolecular complexes of proteins and lipids facilitating specific receptor conformations and distinct distributions, e.g., at the immunological synapse. Thus, a new goal is to develop bioimaging that not only locates proteins in live cells but can also
Suhling, K +6 more
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Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins
Communications Biology, 2021 Zhang, Redington & Gong develop FRET-based ratiometric calcium sensors with pairs of green and red fluorescent proteins. Their best performers, Twitch-GR and Twitch-NR inherit the superior photophysical properties of their constituent fluorescent ...
Diming Zhang +2 more
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Frontiers in Marine Science, 2021 Since the initial discovery of Aqueoria victoria’s green fluorescence off the coast of Washington’s Puget Sound, biofluorescent marine organisms have been found across the globe.
Andrew M. Guarnaccia +10 more
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Using photoactivatable fluorescent protein Dendra2 to track protein movement
BioTechniques, 2007 Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly ...
Dmitriy M. Chudakov +2 more
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Genetically Encoded Biosensors for the Fluorescence Detection of O2 and Reactive O2 Species
Sensors, 2023 The intracellular concentrations of oxygen and reactive oxygen species (ROS) in living cells represent critical information for investigating physiological and pathological conditions.
Marialaura Marchetti +4 more
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BioTechniques, 2015 Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice.
Akifumi Nakagawa +5 more
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Scientific Reports, 2020 In-resin CLEM of Epon embedded samples can greatly simplify the correlation of fluorescent images with electron micrographs. The usefulness of this technique is limited at present by the low number of fluorescent proteins that resist CLEM processing ...
Isei Tanida +5 more
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