Results 11 to 20 of about 1,535 (215)
Fluorescence polarization of green fluorescence protein [PDF]
Proceedings of the National Academy of Sciences, 2002 We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea .
Shinya, Inoué +4 more
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Engineering an efficient and bright split Corynactis californica green fluorescent protein
Scientific Reports, 2021 Split green fluorescent protein (GFP) has been used in a panoply of cellular biology applications to study protein translocation, monitor protein solubility and aggregation, detect protein–protein interactions, enhance protein crystallization, and even ...
Hau B. Nguyen +2 more
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Photoactivation of green fluorescent protein [PDF]
Current Biology, 1997 Considerable insight into many cell biological processes can be obtained by following the turnover of individual protein species in time and space. In living cells, this has been achieved by following fluorescence recovery after local microbeam photobleaching of microinjected fluorescently labelled protein (see, for example, [1xSpindle microtubule ...
Sawin, Kenneth E., Nurse, Paul
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Electrochemical fluorescence switching of enhanced green fluorescent protein
Biosensors and Bioelectronics, 2023 Switchable fluorescent proteins, for which fluorescence can be switched ON and OFF, are widely used for molecule tracking and super resolution imaging. However, the robust use of the switchable fluorescent proteins is still limited as either the switching is not repeatable, or such switching requires irradiation with coupled lasers of different ...
Yang, Y +8 more
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Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein [PDF]
Journal of the American Chemical Society, 2017 Green fluorescent protein, GFP, has revolutionized biology, due to its use in bioimaging. It is widely accepted that the protein environment makes its chromophore fluoresce, whereas the fluorescence is completely lost when the native chromophore is taken out of GFP.
Annette Svendsen +4 more
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Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]
The Journal of Physical Chemistry B, 1999 The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G. +3 more
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BioTechniques, 2023 Fluorescent proteins, such as green fluorescent proteins, are invaluable tools for detecting and quantifying gene expression in high-throughput reporter gene assays. However, they introduce significant inaccuracies in studies involving microaerobiosis or
Lucas Pedraz, Eduard Torrents
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eLife, 2022 Using a neural network to predict how green fluorescent proteins respond to genetic mutations illuminates properties that could help design new proteins.
Grzegorz Kudla, Marcin Plech
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Imaging the Environment of Green Fluorescent Protein [PDF]
Biophysical Journal, 2002 An emerging theme in cell biology is that cell surface receptors need to be considered as part of supramolecular complexes of proteins and lipids facilitating specific receptor conformations and distinct distributions, e.g., at the immunological synapse. Thus, a new goal is to develop bioimaging that not only locates proteins in live cells but can also
Suhling, K +6 more
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Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins
Communications Biology, 2021 Zhang, Redington & Gong develop FRET-based ratiometric calcium sensors with pairs of green and red fluorescent proteins. Their best performers, Twitch-GR and Twitch-NR inherit the superior photophysical properties of their constituent fluorescent ...
Diming Zhang +2 more
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