Results 11 to 20 of about 1,023,137 (333)

A highly photostable and bright green fluorescent protein

Nature Biotechnology, 2022
The low photostability of fluorescent proteins is a limiting factor in many applications of fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) derived from the jellyfish Cytaeis uchidae.
Masahiko Hirano, R. Ando, S. Shimozono, Mayu Sugiyama, N. Takeda, H. Kurokawa, R. Deguchi, Kazuki Endo, K. Haga, Reiko Takai-Todaka, S. Inaura, Yuta Matsumura, H. Hama, Y. Okada, T. Fujiwara, T. Morimoto, Kazuhiko Katayama, A. Miyawaki   +17 more
semanticscholar   +3 more sources

Improving FRET dynamic range with bright green and red fluorescent proteins

Nature Methods, 2012
A variety of genetically encoded reporters use changes in fluorescence (or Förster) resonance energy transfer (FRET) to report on biochemical processes in living cells.
A. Lam, François St-Pierre, Yiyang Gong, Jesse D. Marshall, Paula J. Cranfill, M. A. Baird, M. Mckeown, J. Wiedenmann, M. Davidson, M. Schnitzer, R. Tsien, Michael Z. Lin   +11 more
semanticscholar   +3 more sources

RNA Mimics of Green Fluorescent Protein [PDF]

Science, 2011
Selection for RNAs that bind and activate derivatives of the green fluorescent protein fluorophore yields a wide range of useful spectral properties.
Jeremy S, Paige, Karen Y, Wu, Samie R, Jaffrey   +2 more
openaire   +4 more sources

Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin

Nature, 1997
A. Miyawaki, J. Llopis, R. Heim, J. Mccaffery, J. Adams, Mitsuhiko Ikura, Mitsuhiko Ikura, R. Tsien   +7 more
semanticscholar   +3 more sources

Engineering an efficient and bright split Corynactis californica green fluorescent protein

Scientific Reports, 2021
Split green fluorescent protein (GFP) has been used in a panoply of cellular biology applications to study protein translocation, monitor protein solubility and aggregation, detect protein–protein interactions, enhance protein crystallization, and even ...
Hau B. Nguyen, Thomas C. Terwilliger, Geoffrey S. Waldo   +2 more
doaj   +1 more source

Fluorescence polarization of green fluorescence protein [PDF]

Proceedings of the National Academy of Sciences, 2002
We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea .
Shinya, Inoué, Osamu, Shimomura, Makoto, Goda, Mykhailo, Shribak, P T, Tran   +4 more
openaire   +3 more sources

An easy method for quantification of anaerobic and microaerobic gene expression with fluorescent reporter proteins

BioTechniques, 2023
Fluorescent proteins, such as green fluorescent proteins, are invaluable tools for detecting and quantifying gene expression in high-throughput reporter gene assays. However, they introduce significant inaccuracies in studies involving microaerobiosis or
Lucas Pedraz, Eduard Torrents
doaj   +1 more source

Lighting up protein design

eLife, 2022
Using a neural network to predict how green fluorescent proteins respond to genetic mutations illuminates properties that could help design new proteins.
Grzegorz Kudla, Marcin Plech
doaj   +1 more source

Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins

Communications Biology, 2021
Zhang, Redington & Gong develop FRET-based ratiometric calcium sensors with pairs of green and red fluorescent proteins. Their best performers, Twitch-GR and Twitch-NR inherit the superior photophysical properties of their constituent fluorescent ...
Diming Zhang, Emily Redington, Yiyang Gong   +2 more
doaj   +1 more source

Discovery and Characterization of a Bilirubin Inducible Green Fluorescent Protein From the Moray Eel Gymnothorax zonipectis

Frontiers in Marine Science, 2021
Since the initial discovery of Aqueoria victoria’s green fluorescence off the coast of Washington’s Puget Sound, biofluorescent marine organisms have been found across the globe.
Andrew M. Guarnaccia, Andrew M. Guarnaccia, Sara Rose Krivoshik, Sara Rose Krivoshik, John S. Sparks, John S. Sparks, David F. Gruber, David F. Gruber, David F. Gruber, Jean P. Gaffney, Jean P. Gaffney   +10 more
doaj   +1 more source