Results 21 to 30 of about 1,535 (215)

Discovery and Characterization of a Bilirubin Inducible Green Fluorescent Protein From the Moray Eel Gymnothorax zonipectis

Frontiers in Marine Science, 2021
Since the initial discovery of Aqueoria victoria’s green fluorescence off the coast of Washington’s Puget Sound, biofluorescent marine organisms have been found across the globe.
Andrew M. Guarnaccia, Andrew M. Guarnaccia, Sara Rose Krivoshik, Sara Rose Krivoshik, John S. Sparks, John S. Sparks, David F. Gruber, David F. Gruber, David F. Gruber, Jean P. Gaffney, Jean P. Gaffney   +10 more
doaj   +1 more source

Genetically Encoded Biosensors for the Fluorescence Detection of O₂ and Reactive O₂ Species

Sensors, 2023
The intracellular concentrations of oxygen and reactive oxygen species (ROS) in living cells represent critical information for investigating physiological and pathological conditions.
Marialaura Marchetti, Luca Ronda, Monica Cozzi, Stefano Bettati, Stefano Bruno   +4 more
doaj   +1 more source

Using photoactivatable fluorescent protein Dendra2 to track protein movement

BioTechniques, 2007
Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly ...
Dmitriy M. Chudakov, Sergey Lukyanov, Konstantin A. Lukyanov   +2 more
doaj   +1 more source

A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins

BioTechniques, 2015
Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice.
Akifumi Nakagawa, Kate Von Alt, Keith D. Lillemoe, Carlos Fernández-del Castillo, Andrew L. Warshaw, Andrew S. Liss   +5 more
doaj   +1 more source

Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins

Scientific Reports, 2020
In-resin CLEM of Epon embedded samples can greatly simplify the correlation of fluorescent images with electron micrographs. The usefulness of this technique is limited at present by the low number of fluorescent proteins that resist CLEM processing ...
Isei Tanida, Yoko Furuta, Junji Yamaguchi, Soichiro Kakuta, Juan Alejandro Oliva Trejo, Yasuo Uchiyama   +5 more
doaj   +1 more source

A Bright, Nontoxic, and Non-aggregating red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

Frontiers in Cell and Developmental Biology, 2022
Red fluorescent proteins are useful as morphological markers in neurons, often complementing green fluorescent protein-based probes of neuronal activity. However, commonly used red fluorescent proteins show aggregation and toxicity in neurons or are dim.
Lin Ning, Yang Geng, Yang Geng, Matthew Lovett-Barron, Xiaoman Niu, Mengying Deng, Liang Wang, Niloufar Ataie, Alex Sens, Ho-Leung Ng, Shoudeng Chen, Karl Deisseroth, Michael Z. Lin, Michael Z. Lin, Jun Chu   +14 more
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Green fluorescent protein: A perspective [PDF]

Protein Science, 2011
AbstractA brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ...
openaire   +2 more sources

Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum

BioTechniques, 2006
O6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins can be specifically and covalently labeled with fluorescent O6-benzylguanine (O6-BG) derivatives for multicolor live cell imaging approaches.
Antje Keppler, Claudio Arrivoli, Lucia Sironi, Jan Ellenberg   +3 more
doaj   +1 more source

Red fluorescent proteins engineered from green fluorescent proteins

Proceedings of the National Academy of Sciences, 2023
Fluorescent proteins (FPs) form a fluorophore through autocatalysis from three consecutive amino acid residues within a polypeptide chain. The two major groups, green FPs (GFPs) and red FPs (RFPs), have distinct fluorophore structures; RFPs have an extended π-conjugation system with an additional double bond.
Hiromi Imamura, Shiho Otsubo, Mizuho Nishida, Norihiro Takekawa, Katsumi Imada   +4 more
openaire   +2 more sources

Fluorescent proteins as tools to aid protein production

Microbial Cell Factories, 2005
Fluorescent proteins are genetically encoded, highly versatile reporters useful for monitoring various aspects of recombinant protein production. In addition to the widely popular green fluorescent protein (GFP) from Aequorea victoria, a variety of other
Su Wei
doaj   +1 more source