Results 21 to 30 of about 3,922,099 (302)

Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein [PDF]

open access: yesJournal of the American Chemical Society, 2017
Green fluorescent protein, GFP, has revolutionized biology, due to its use in bioimaging. It is widely accepted that the protein environment makes its chromophore fluoresce, whereas the fluorescence is completely lost when the native chromophore is taken out of GFP.
Annette Svendsen   +4 more
openaire   +3 more sources

Lighting up protein design

open access: yeseLife, 2022
Using a neural network to predict how green fluorescent proteins respond to genetic mutations illuminates properties that could help design new proteins.
Grzegorz Kudla, Marcin Plech
doaj   +1 more source

Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]

open access: yesThe Journal of Physical Chemistry B, 1999
The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G.   +3 more
openaire   +2 more sources

Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins

open access: yesCommunications Biology, 2021
Zhang, Redington & Gong develop FRET-based ratiometric calcium sensors with pairs of green and red fluorescent proteins. Their best performers, Twitch-GR and Twitch-NR inherit the superior photophysical properties of their constituent fluorescent ...
Diming Zhang   +2 more
doaj   +1 more source

Discovery and Characterization of a Bilirubin Inducible Green Fluorescent Protein From the Moray Eel Gymnothorax zonipectis

open access: yesFrontiers in Marine Science, 2021
Since the initial discovery of Aqueoria victoria’s green fluorescence off the coast of Washington’s Puget Sound, biofluorescent marine organisms have been found across the globe.
Andrew M. Guarnaccia   +10 more
doaj   +1 more source

Imaging the Environment of Green Fluorescent Protein [PDF]

open access: yesBiophysical Journal, 2002
An emerging theme in cell biology is that cell surface receptors need to be considered as part of supramolecular complexes of proteins and lipids facilitating specific receptor conformations and distinct distributions, e.g., at the immunological synapse. Thus, a new goal is to develop bioimaging that not only locates proteins in live cells but can also
Suhling, K   +6 more
openaire   +4 more sources

Screening by coral green fluorescent protein (GFP)-like chromoproteins supports a role in photoprotection of zooxanthellae [PDF]

open access: yes, 2013
Green fluorescent protein (GFP)-like pigments are responsible for the vivid colouration of many reef-building corals and have been proposed to act as photoprotectants.
Smith, E.G.   +9 more
core   +1 more source

Using photoactivatable fluorescent protein Dendra2 to track protein movement

open access: yesBioTechniques, 2007
Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly ...
Dmitriy M. Chudakov   +2 more
doaj   +1 more source

Genetically Encoded Biosensors for the Fluorescence Detection of O2 and Reactive O2 Species

open access: yesSensors, 2023
The intracellular concentrations of oxygen and reactive oxygen species (ROS) in living cells represent critical information for investigating physiological and pathological conditions.
Marialaura Marchetti   +4 more
doaj   +1 more source

Bio-optimized energy transfer in densely packed fluorescent protein enables near-maximal luminescence and solid-state lasers [PDF]

open access: yes, 2014
This work was supported by the US National Science Foundation (ECCS- 1101947), National Institutes of Health (P41EB015903, R21EB013761), Department of Defense (FA9550-11-1-0331), and the Korea National Research Foundation (R31-2008-000-10071-0).
Gather, Malte Christian, Yun, Seok Hyun
core   +1 more source

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