Results 21 to 30 of about 510,204 (262)

A Bright, Nontoxic, and Non-aggregating red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

open access: yesFrontiers in Cell and Developmental Biology, 2022
Red fluorescent proteins are useful as morphological markers in neurons, often complementing green fluorescent protein-based probes of neuronal activity. However, commonly used red fluorescent proteins show aggregation and toxicity in neurons or are dim.
Lin Ning   +14 more
doaj   +1 more source

Green fluorescent protein: A perspective [PDF]

open access: yesProtein Science, 2011
AbstractA brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ...
openaire   +2 more sources

Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum

open access: yesBioTechniques, 2006
O6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins can be specifically and covalently labeled with fluorescent O6-benzylguanine (O6-BG) derivatives for multicolor live cell imaging approaches.
Antje Keppler   +3 more
doaj   +1 more source

Fluorescent proteins as tools to aid protein production

open access: yesMicrobial Cell Factories, 2005
Fluorescent proteins are genetically encoded, highly versatile reporters useful for monitoring various aspects of recombinant protein production. In addition to the widely popular green fluorescent protein (GFP) from Aequorea victoria, a variety of other
Su Wei
doaj   +1 more source

Fluorescent proteins reveal what trypanosomes get up to inside the tsetse fly

open access: yesParasites & Vectors, 2019
The discovery and development of fluorescent proteins for the investigation of living cells and whole organisms has been a major advance in biomedical research.
Wendy Gibson, Lori Peacock
doaj   +1 more source

R26R-GR: a Cre-activable dual fluorescent protein reporter mouse. [PDF]

open access: yesPLoS ONE, 2012
Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level.
You-Tzung Chen   +21 more
doaj   +1 more source

Green Fluorescent Protein: discover, structure, purification, applications, and future prospects

open access: yesJournal of Scientific Research in Science, 2023
Green fluorescent protein (GFP) Firstly discovered from the jellyfish Aequorea victoriaand it was widely used in biological science as a universal fluorescent tag.
Hend A. Anter   +2 more
doaj   +1 more source

Getting to grips with ammonium

open access: yeseLife, 2013
A fluorescent sensor that can monitor levels of extracellular ammonium has been made by using a fused green fluorescent protein to detect conformational changes in ammonium transport proteins.
Yi Wang, Jonathan A Javitch
doaj   +1 more source

Fluorescence color diversity of great barrier reef corals [PDF]

open access: yesJournal of Innovative Optical Health Sciences, 2015
A group of variously colored proteins belonging to the green fluorescent protein (GFP) family are responsible for coloring coral tissues. Corals of the Great Barrier Reef were studied with the custom-built fiber laser fluorescence spectrometers. Spectral
Grigory Lapshin   +8 more
doaj   +1 more source

A modular toolset of phiC31-based fluorescent protein tagging vectors for Drosophila

open access: yesFly, 2019
The Drosophila transgenic technology and fluorescent protein fusions are powerful tools to analyze protein expression patterns, subcellular localization and protein dynamics.
Jun Luo, Pingping Shen, Jiong Chen
doaj   +1 more source

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