Results 21 to 30 of about 1,023,137 (333)

Genetically Encoded Biosensors for the Fluorescence Detection of O₂ and Reactive O₂ Species

Sensors, 2023
The intracellular concentrations of oxygen and reactive oxygen species (ROS) in living cells represent critical information for investigating physiological and pathological conditions.
Marialaura Marchetti, Luca Ronda, Monica Cozzi, Stefano Bettati, Stefano Bruno   +4 more
doaj   +1 more source

Using photoactivatable fluorescent protein Dendra2 to track protein movement

BioTechniques, 2007
Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly ...
Dmitriy M. Chudakov, Sergey Lukyanov, Konstantin A. Lukyanov   +2 more
doaj   +1 more source

A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins

BioTechniques, 2015
Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice.
Akifumi Nakagawa, Kate Von Alt, Keith D. Lillemoe, Carlos Fernández-del Castillo, Andrew L. Warshaw, Andrew S. Liss   +5 more
doaj   +1 more source

Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins

Scientific Reports, 2020
In-resin CLEM of Epon embedded samples can greatly simplify the correlation of fluorescent images with electron micrographs. The usefulness of this technique is limited at present by the low number of fluorescent proteins that resist CLEM processing ...
Isei Tanida, Yoko Furuta, Junji Yamaguchi, Soichiro Kakuta, Juan Alejandro Oliva Trejo, Yasuo Uchiyama   +5 more
doaj   +1 more source

A Bright, Nontoxic, and Non-aggregating red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

Frontiers in Cell and Developmental Biology, 2022
Red fluorescent proteins are useful as morphological markers in neurons, often complementing green fluorescent protein-based probes of neuronal activity. However, commonly used red fluorescent proteins show aggregation and toxicity in neurons or are dim.
Lin Ning, Yang Geng, Yang Geng, Matthew Lovett-Barron, Xiaoman Niu, Mengying Deng, Liang Wang, Niloufar Ataie, Alex Sens, Ho-Leung Ng, Shoudeng Chen, Karl Deisseroth, Michael Z. Lin, Michael Z. Lin, Jun Chu   +14 more
doaj   +1 more source

Photoactivation of green fluorescent protein [PDF]

Current Biology, 1997
Considerable insight into many cell biological processes can be obtained by following the turnover of individual protein species in time and space. In living cells, this has been achieved by following fluorescence recovery after local microbeam photobleaching of microinjected fluorescently labelled protein (see, for example, [1xSpindle microtubule ...
Sawin, Kenneth E., Nurse, Paul
openaire   +2 more sources

Electrochemical fluorescence switching of enhanced green fluorescent protein

Biosensors and Bioelectronics, 2023
Switchable fluorescent proteins, for which fluorescence can be switched ON and OFF, are widely used for molecule tracking and super resolution imaging. However, the robust use of the switchable fluorescent proteins is still limited as either the switching is not repeatable, or such switching requires irradiation with coupled lasers of different ...
Yang, Y, Fan, S, Webb, JA, Ma, Y, Goyette, J, Chen, X, Gaus, K, Tilley, RD, Gooding, JJ   +8 more
openaire   +3 more sources

Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae.

PLoS ONE, 2013
Fluorescent protein fusions are a powerful tool to monitor the localization and trafficking of proteins. Such studies are particularly easy to carry out in the budding yeast Saccharomyces cerevisiae due to the ease with which tags can be introduced into ...
Sidae Lee, Wendell A Lim, Kurt S Thorn, Kurt S Thorn   +3 more
doaj   +1 more source

Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum

BioTechniques, 2006
O6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins can be specifically and covalently labeled with fluorescent O6-benzylguanine (O6-BG) derivatives for multicolor live cell imaging approaches.
Antje Keppler, Claudio Arrivoli, Lucia Sironi, Jan Ellenberg   +3 more
doaj   +1 more source

Direction dependent mechanical unfolding and Green Fluorescent Protein as a force sensor [PDF]

, 2011
An Ising--like model of proteins is used to investigate the mechanical unfolding of the Green Fluorescent Protein along different directions. When the protein is pulled from its ends, we recover the major and minor unfolding pathways observed in ...
Caraglio, M., Imparato, A., Pelizzola, A.   +2 more
core   +2 more sources