Results 41 to 50 of about 1,535 (215)

A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins

BioTechniques, 2017
Protocol Summary Here we present an ethanol fixation protocol that allows for the direct visualization of the natural fluorescence of reporter proteins while maintaining excellent tissue histology.
Akifumi Nakagawa, Kate Von Alt, Keith D. Lillemoe, Carlos Fernández-del Castillo, Andrew L. Warshaw, Andrew S. Liss   +5 more
doaj   +1 more source

The molecular structure of green fluorescent protein [PDF]

Nature Biotechnology, 1996
The crystal structure of recombinant wild-type green fluorescent protein (GFP) has been solved to a resolution of 1.9 A by multiwavelength anomalous dispersion phasing methods. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder.
F, Yang, L G, Moss, G N, Phillips
openaire   +2 more sources

Bright Green Biofluorescence in Sharks Derives from Bromo-Kynurenine Metabolism

iScience, 2019
Summary: Although in recent years there has been an increased awareness of the widespread nature of biofluorescence in the marine environment, the diversity of the molecules responsible for this luminescent phenotype has been mostly limited to green ...
Hyun Bong Park, Yick Chong Lam, Jean P. Gaffney, James C. Weaver, Sara Rose Krivoshik, Randy Hamchand, Vincent Pieribone, David F. Gruber, Jason M. Crawford   +8 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

A family of GFP-like proteins with different spectral properties in lancelet Branchiostoma floridae

Biology Direct, 2008
Background Members of the green fluorescent protein (GFP) family share sequence similarity and the 11-stranded β-barrel fold. Fluorescence or bright coloration, observed in many members of this family, is enabled by the intrinsic properties of the ...
Mushegian Arcady, Ma Limei, Cook Malcolm, Baumann Diana, Sanders Erik, Schwartz Joel, Yu C Ron   +6 more
doaj   +1 more source

Sea as a color palette: the ecology and evolution of fluorescence

Zoological Letters, 2020
Fluorescence and luminescence are widespread optical phenomena exhibited by organisms living in terrestrial and aquatic environments. While many underlying mechanistic features have been identified and characterized at the molecular and cellular levels ...
Marie-Lyne Macel, Filomena Ristoratore, Annamaria Locascio, Antonietta Spagnuolo, Paolo Sordino, Salvatore D’Aniello   +5 more
doaj   +1 more source

Variants of Green Fluorescent Protein GFPxm [PDF]

Marine Biotechnology, 2006
As research progresses, fluorescent proteins useful for optical marking will evolve toward brighter, monomeric forms that are more diverse in color. We previously reported a new fluorescent protein from Aequorea macrodactyla, GFPxm, that exhibited many characteristics similar to wild-type green fluorescent protein (GFP).
Luo, W. X., Cheng, T., Guan, B. Q., Li, S. W., Miao, J., Zhang, J., Xia, N. S.   +6 more
openaire   +3 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Selective Immobilization of Fluorescent Proteins for the Fabrication of Photoactive Materials

Molecules, 2019
The immobilization of fluorescent proteins is a key technology enabling to fabricate a new generation of photoactive materials with potential technological applications. Herein we have exploited superfolder green (sGFP) and red (RFP) fluorescent proteins
Ana I. Benítez-Mateos, Ehsan Mehravar, Susana Velasco-Lozano, Radmila Tomovska, Luca Salassa, Fernando López-Gallego   +5 more
doaj   +1 more source

Photoacid Dynamics in the Green Fluorescent Protein

Annual Review of Physical Chemistry, 2023
The photoacid dynamics of fluorescent proteins include both electronic excited- and ground-state mechanisms of proton transfer. The associated characteristic timescales of these reactions range over many orders of magnitude, and the tunneling, barrier crossing, and relevant thermodynamics have in certain cases been linked to coherent nuclear motion ...
Jasper J, van Thor, Paul M, Champion
openaire   +2 more sources