Results 41 to 50 of about 1,023,137 (333)

A faster way to make GFP-based biosensors: Two new transposons for creating multicolored libraries of fluorescent fusion proteins [PDF]

open access: yes, 2004
Background There are now several ways to generate fluorescent fusion proteins by randomly inserting DNA encoding the Green Fluorescent Protein (GFP) into another protein's coding sequence. These approaches can be used to map regions in a protein that are
Douglas L Sheridan, Thomas E Hughes
core   +2 more sources

Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting

open access: yesScientific Reports, 2016
Many genetically encoded biosensors use Förster resonance energy transfer (FRET) to dynamically report biomolecular activities. While pairs of cyan and yellow fluorescent proteins (FPs) are most commonly used as FRET partner fluorophores, respectively ...
B. Bajar   +8 more
semanticscholar   +1 more source

Fluorescent proteins reveal what trypanosomes get up to inside the tsetse fly

open access: yesParasites & Vectors, 2019
The discovery and development of fluorescent proteins for the investigation of living cells and whole organisms has been a major advance in biomedical research.
Wendy Gibson, Lori Peacock
doaj   +1 more source

Watching gene expression in color. [PDF]

open access: yes, 2019
A combination of two fluorescent proteins with different half-lives allows gene expression to be followed with improved time ...
Bates   +10 more
core   +2 more sources

Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]

open access: yesThe Journal of Physical Chemistry B, 1999
The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G.   +3 more
openaire   +2 more sources

Studies of Single-Molecule Dynamics in Microorganisms [PDF]

open access: yes, 2019
Fluorescence microscopy is one of the most extensively used techniques in the life sciences. Considering the non-invasive sample preparation, enabling live-cell compliant imaging, and the specific fluorescence labeling, allowing for a specific visualization
Turkowyd, Bartosz Daniel
core   +1 more source

Genetically encoded fluorescent redox probes. [PDF]

open access: yes, 2013
Redox processes are involved in almost every cell of the body as a consequence of aerobic life. In the past decades, redox biology has been increasingly recognized as one of the key themes in cell signaling.
Ai, Hui-Wang, Ren, Wei
core   +3 more sources

Fluorescent protein markers to tag collagenous proteins: The paradigm of procollagen VII [PDF]

open access: yes, 2009
Fluorescent proteins are powerful markers allowing tracking expression, intracellular localization, and translocation of tagged proteins but their effects on the structure and assembly of complex extracellular matrix proteins has not been investigated ...
Chung, Hye J.   +3 more
core   +2 more sources

Functional divergence of microtubule-associated TPX2 family members in Arabidopsis thaliana [PDF]

open access: yes, 2020
TPX2 (Targeting Protein for Xklp2) is an evolutionary conserved microtubule-associated protein important for microtubule nucleation and mitotic spindle assembly.
Demidov, Dmitri   +9 more
core   +1 more source

Photoactivatable Green Fluorescent Protein-Tubulin [PDF]

open access: yes, 2010
Direct observations of live cells expressing fluorescently tagged tubulin have led to important advances in our understanding of mitosis. A limitation of this approach is that all of the cells' microtubules are fluorescent and thus observation of the behavior of specific subsets of microtubules is precluded.
U Serdar, Tulu   +2 more
openaire   +2 more sources

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