Results 61 to 70 of about 510,204 (262)
RNA Mimics of Green Fluorescent Protein [PDF]
Science, 2011 Selection for RNAs that bind and activate derivatives of the green fluorescent protein fluorophore yields a wide range of useful spectral properties.
Jeremy S, Paige +2 more
openaire +2 more sources
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Sub-cellular trafficking of phytochemicals explored using auto-fluorescent compounds in maize cells
BMC Plant Biology, 2003 Background Little is known regarding the trafficking mechanisms of small molecules within plant cells. It remains to be established whether phytochemicals are transported by pathways similar to those used by proteins, or whether the expansion of ...
Grotewold Erich +2 more
doaj +1 more source
FEBS Letters, EarlyView.The mycobacterial CIII‐CIV respiratory supercomplex is an obligate assembly, encompassing several subunits of unknown functions. We have characterized the intracellular subunit AscF, and show that it is unlikely to be a sensor for metals or nucleotides, but is required for growth on nonfermentable energy sources, and likely works as an adapter for ...
Eni Rile +8 more
wiley +1 more source
Fluorescent proteins such as eGFP lead to catalytic oxidative stress in cells
Redox Biology, 2017 Fluorescent proteins are an important tool that has become omnipresent in life sciences research. They are frequently used for localization of proteins and monitoring of cells [1,2].
Douglas Ganini +7 more
doaj +1 more source
Green Fluorescent Protein Glows Gold [PDF]
Cell, 2008 The awarding of this year's Nobel Prize in Chemistry to Osamu Shimomura, Martin Chalfie, and Roger Tsien for their discovery and development of green fluorescent protein earns this humble jellyfish protein a place of honor in the biology research hall of fame.
openaire +2 more sources
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
FEBS Letters, EarlyView.GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Proton Pathways in Green Fluorescence Protein [PDF]
Biophysical Journal, 2005 Proton pathways in green fluorescent protein (GFP) are more extended than previously reported. In the x-ray data of wild-type GFP, a two-step exit pathway exists from the active site to the protein surface, controlled by a threonine switch. A proton entry pathway begins at a glutamate-lysine cluster around Glu-5, and extends all the way to the buried ...
openaire +2 more sources
Discerning protein pools by selective staining with self‐labeling tags
FEBS Letters, EarlyView.Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source
Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples.
PLoS ONE, 2007 BackgroundFluorescence Resonance Energy Transfer (FRET) between the green fluorescent protein (GFP) variants CFP and YFP is widely used for the detection of protein-protein interactions.
Joachim Goedhart +4 more
doaj +1 more source